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      Molecular detection and point-of-care testing in Ebola virus disease and other threats: a new global public health framework to stop outbreaks

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      Expert Review of Molecular Diagnostics

      Informa UK Limited

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          Most cited references 12

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          Development of a panel of recombinase polymerase amplification assays for detection of biothreat agents.

          Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents. The panel included RPAs for Francisella tularensis, Yersinia pestis, Bacillus anthracis, variola virus, and reverse transcriptase RPA (RT-RPA) assays for Rift Valley fever virus, Ebola virus, Sudan virus, and Marburg virus. Their analytical sensitivities ranged from 16 to 21 molecules detected (probit analysis) for the majority of RPA and RT-RPA assays. A magnetic bead-based total nucleic acid extraction method was combined with the RPAs and tested using inactivated whole organisms spiked into plasma. The RPA showed comparable sensitivities to real-time RCR assays in these extracts. The run times of the assays at 42°C ranged from 6 to 10 min, and they showed no cross-detection of any of the target genomes of the panel nor of the human genome. The RPAs therefore seem suitable for the implementation of syndromic panels onto microfluidic platforms.
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            Multicolored silver nanoparticles for multiplexed disease diagnostics: distinguishing dengue, yellow fever, and Ebola viruses.

            Rapid point-of-care (POC) diagnostic devices are needed for field-forward screening of severe acute systemic febrile illnesses. Multiplexed rapid lateral flow diagnostics have the potential to distinguish among multiple pathogens, thereby facilitating diagnosis and improving patient care. Here, we present a platform for multiplexed pathogen detection using multi-colored silver nanoplates. This design requires no external excitation source and permits multiplexed analysis in a single channel, facilitating integration and manufacturing.
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              Laboratory test support for ebola patients within a high-containment facility.

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                Author and article information

                Journal
                Expert Review of Molecular Diagnostics
                Expert Review of Molecular Diagnostics
                Informa UK Limited
                1473-7159
                1744-8352
                September 07 2015
                October 03 2015
                September 14 2015
                October 03 2015
                : 15
                : 10
                : 1245-1259
                10.1586/14737159.2015.1079776
                © 2015

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