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      Primary structure requirements for the binding of human high molecular weight kininogen to plasma prekallikrein and factor XI.

      The Journal of Biological Chemistry
      Amino Acid Sequence, Chromatography, Affinity, Factor XI, metabolism, Humans, Kallikreins, blood, Kaolin, pharmacology, Kininogens, Mathematics, Peptide Mapping, Prekallikrein

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          Abstract

          We recently identified residues 185-224 of the light chain of human high molecular weight kininogen (HMWK) as the binding site for plasma prekallikrein (Tait, J.F., and Fujikawa, K. (1986) J. Biol. Chem. 261, 15396-15401). In the present study, we have further defined the primary structure requirements for binding of HMWK to factor XI and prekallikrein. In a competitive fluorescence polarization binding assay, a 31-residue synthetic peptide (residues 194-224 of the HMWK light chain) bound to prekallikrein with a Kd of 20 +/- 6 nM, indistinguishable from the previously determined value of 18 +/- 5 nM for the light chain. We also prepared three shorter synthetic peptides corresponding to different portions of the 31-residue peptide (residues 205-224, 212-224, and 194-211), but these peptides bound to prekallikrein more than 100-fold more weakly. Factor XI also bound to the same region of the HMWK light chain, but at least 58 residues (185-242) were required for optimal binding (Kd = 69 +/- 4 nM for the light chain; Kd = 130 +/- 50 nM for residues 185-242). The four synthetic peptides inhibited kaolin-activated clotting of blood plasma with potencies paralleling their affinities for prekallikrein and factor XI. Peptide 194-224 can also be used for rapid affinity purification of prekallikrein and factor XI from plasma.

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          Author and article information

          Journal
          3650269
          10.1016/S0021-9258(18)60859-2

          Chemistry
          Amino Acid Sequence,Chromatography, Affinity,Factor XI,metabolism,Humans,Kallikreins,blood,Kaolin,pharmacology,Kininogens,Mathematics,Peptide Mapping,Prekallikrein

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