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      Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization.

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          Abstract

          Dengue viral antigens have been demonstrated in several types of naturally infected human tissues, but little is known of whether these same tissues have detectable viral RNA. We studied tissue specimens from patients with serologically or virologically confirmed dengue infections by immunohistochemistry (IHC) and in situ hybridization (ISH), to localize viral antigen and RNA, respectively. IHC was performed on specimens obtained from 5 autopsies and 24 biopsies and on 20 blood-clot samples. For ISH, antisense riboprobes to the dengue E gene were applied to tissue specimens in which IHC was positive. Viral antigens were demonstrated in Kupffer and sinusoidal endothelial cells of the liver; macrophages, multinucleated cells, and reactive lymphoid cells in the spleen; macrophages and vascular endothelium in the lung; kidney tubules; and monocytes and lymphocytes in blood-clot samples. Positive-strand viral RNA was detected in the same IHC-positive cells found in the spleen and blood-clot samples. The strong, positive ISH signal in these cells indicated a high copy number of viral RNA, suggesting replication.

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          Author and article information

          Journal
          J Infect Dis
          The Journal of infectious diseases
          University of Chicago Press
          0022-1899
          0022-1899
          Apr 15 2004
          : 189
          : 8
          Affiliations
          [1 ] Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
          Article
          JID31407
          10.1086/383043
          15073678
          6754dbea-ac3c-421d-bdfb-892979791573
          History

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