There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Introduction
In recent years there has been increasing evidence that a resuscitation strategy with
different fluids can have widely divergent impacts on the immune response, neutrophil
activation and tissue injury. This prospective study was undertaken to determine the
neutrophil oxidative burst in the swine model during an acute normovolemic hemodilution
(ANH) procedure with hydroxyethyl starch.
Methods
Twelve pigs were anesthetized, instrumented and randomized into two groups: control
and hemodilution (H). The control group was only anesthetized and instrumented while
animals in the ANH group were submitted to acute normovolemic hemodilution to a target
hematocrit of 15% with volume replacement performed with hydroxyethyl starch 130/0.4
at a 1:1 ratio. The withdrawn blood was returned to the animals 120 minutes after
the end of hemodilution. Neutrophil oxidative burst was performed with blood samples
collected at the femoral vein at the following time points: before ANH (baseline),
after instrumentation (INST), immediately after ANH (H), 60 minutes after ANH (60H),
120 minutes after ANH (120H), 60 minutes after blood infusion (60BI) and 120 minutes
after blood infusion (120BI), and determined with a flow cytometer. Spontaneous and
stimulated oxidative burst activation of neutrophils were performed with dichlorofluorescein
diacetate and phorbol myristate acetate. Statistical analyses were performed using
one-way analysis of variance followed by a Dunnett test or t test. A P value of 0.05
was considered statistically significant.
Results
Spontaneous oxidative burst activity in group H increased significantly from baseline
(30.19 ± 4.79) to H (57.45 ± 9.86) and 60H (56.26 ± 14.64) (P < 0.01) while the control
group did not present significant variation. Between groups there were significant
differences at H (ANH = 57.45 ± 9.86; control = 23.18 ± 7.16; P = 0.0007), 60H (ANH
= 56.26 ± 14.64; control = 34.53 ± 9.06; P = 0.0225), 120H (ANH = 43.59 ± 5.46; control
= 28.65 ± 10.44; P = 0.0220) and 60BI (ANH = 38.60 ± 1.85; control = 25.59 ± 8.12;
P = 0.0082).
Conclusion
ANH with hydroxyethyl starch influences oxidative burst activity under experimental
conditions.