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      Neutrophil oxidative burst evaluation during acute normovolemic hemodilution: preliminary results

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      1 , 1 , 1 , 1 , 1 , 1
      Critical Care
      BioMed Central
      27th International Symposium on Intensive Care and Emergency Medicine
      27-30 March 2007

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          Abstract

          Introduction In recent years there has been increasing evidence that a resuscitation strategy with different fluids can have widely divergent impacts on the immune response, neutrophil activation and tissue injury. This prospective study was undertaken to determine the neutrophil oxidative burst in the swine model during an acute normovolemic hemodilution (ANH) procedure with hydroxyethyl starch. Methods Twelve pigs were anesthetized, instrumented and randomized into two groups: control and hemodilution (H). The control group was only anesthetized and instrumented while animals in the ANH group were submitted to acute normovolemic hemodilution to a target hematocrit of 15% with volume replacement performed with hydroxyethyl starch 130/0.4 at a 1:1 ratio. The withdrawn blood was returned to the animals 120 minutes after the end of hemodilution. Neutrophil oxidative burst was performed with blood samples collected at the femoral vein at the following time points: before ANH (baseline), after instrumentation (INST), immediately after ANH (H), 60 minutes after ANH (60H), 120 minutes after ANH (120H), 60 minutes after blood infusion (60BI) and 120 minutes after blood infusion (120BI), and determined with a flow cytometer. Spontaneous and stimulated oxidative burst activation of neutrophils were performed with dichlorofluorescein diacetate and phorbol myristate acetate. Statistical analyses were performed using one-way analysis of variance followed by a Dunnett test or t test. A P value of 0.05 was considered statistically significant. Results Spontaneous oxidative burst activity in group H increased significantly from baseline (30.19 ± 4.79) to H (57.45 ± 9.86) and 60H (56.26 ± 14.64) (P < 0.01) while the control group did not present significant variation. Between groups there were significant differences at H (ANH = 57.45 ± 9.86; control = 23.18 ± 7.16; P = 0.0007), 60H (ANH = 56.26 ± 14.64; control = 34.53 ± 9.06; P = 0.0225), 120H (ANH = 43.59 ± 5.46; control = 28.65 ± 10.44; P = 0.0220) and 60BI (ANH = 38.60 ± 1.85; control = 25.59 ± 8.12; P = 0.0082). Conclusion ANH with hydroxyethyl starch influences oxidative burst activity under experimental conditions.

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          Author and article information

          Conference
          Crit Care
          Crit Care
          Critical Care
          BioMed Central
          1364-8535
          1466-609X
          2007
          22 March 2007
          : 11
          : Suppl 2
          : P40
          Affiliations
          [1 ]University of São Paulo Medical School, São Paulo, Brazil
          Article
          cc5200
          10.1186/cc5200
          4095094
          67643cf6-d200-463f-878f-39e172fb933d
          Copyright © 2007 BioMed Central Ltd.
          27th International Symposium on Intensive Care and Emergency Medicine
          Brussels, Belgium
          27-30 March 2007
          History
          Categories
          Poster Presentation

          Emergency medicine & Trauma
          Emergency medicine & Trauma

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