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      A New Computerized Biomechanical Perfusion Model for ex vivo Study of Fluid Mechanical Forces in Intact Conduit Vessels

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          Abstract

          We have developed a new computerized biomechanical ex vivo perfusion system for intact conduit vessels in which a wide range of combinations of intraluminal pressure, fluid flow and shear stress could be set and maintained at target levels in mammalian conduit vessels under controlled metabolic conditions. Mean wall shear stress is calculated using the formula:τ = 1/2 * (ΔP/L)<sup>3/4</sup> * (8ηQ/Π)<sup>1/4</sup>.Accuracy of the wall shear stress calculation was validated by ultrasonographic imaging of the vessel radius. In a series of simulation experiments, the hemodynamic homeostasis functions of the system were challenged by generating a wide range of vascular resistance in artificial vessels and by pharmacologically induced changes in vascular tone in intact human vessels. Despite rapid changes in vessel resistance, shear stress and pressure, or flow and pressure were maintained well at target levels. Shear- and pressure-stimulated production of the vasodilator prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) was used to validate the biological relevance of the model. PGE<sub>2</sub> release was significantly more stimulated by high (25 dyn/cm<sup>2</sup>) compared to low (<4 dyn/cm<sup>2</sup>) shear (ANOVA, p = 0.012). High compared to low intraluminal pressure depressed the production of PGE<sub>2</sub> (ANOVA, p = 0.019). In summary, the computerized perfusion model appears to offer new possibilities of investigating the complex interplay between fluid mechanics and the vascular wall.

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          Most cited references 2

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          Apparatus for subjecting living cells to fluid shear stress.

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            Influence of pressure, flow rate, and pulsatility on release of 6-keto-PGF and thromboxane B in ex vivo[mdash ]perfused canine veins

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              Author and article information

              Journal
              JVR
              J Vasc Res
              10.1159/issn.1018-1172
              Journal of Vascular Research
              S. Karger AG
              1018-1172
              1423-0135
              1999
              February 1999
              01 March 1999
              : 36
              : 1
              : 68-78
              Affiliations
              Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Östra, Göteborg University, Göteborg, Sweden
              Article
              25627 J Vasc Res 1999;36:68–78
              10.1159/000025627
              10050075
              © 1999 S. Karger AG, Basel

              Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

              Page count
              Figures: 6, References: 24, Pages: 11
              Categories
              Research Paper

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