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      Detection of parathion and patulin by quartz-crystal microbalance functionalized by the photonics immobilization technique.

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          Abstract

          Oriented antibodies are tethered on the gold surface of a quartz crystal microbalance through the photonics immobilization technique so that limit of detection as low as 50 nM and 140 nM are achieved for parathion and patulin, respectively. To make these small analytes detectable by the microbalance, they have been weighed down through a "sandwich protocol" with a second antibody. The specificity against the parathion has been tested by checking the immunosensor response to a mixture of compounds similar to parathion, whereas the specificity against the patulin has been tested with a real sample from apple puree. In both cases, the results are more than satisfactory suggesting interesting outlook for the proposed device.

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          Author and article information

          Journal
          Biosens Bioelectron
          Biosensors & bioelectronics
          Elsevier BV
          1873-4235
          0956-5663
          May 15 2015
          : 67
          Affiliations
          [1 ] CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy.
          [2 ] Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco, Via P. Vitiello, 108, Scafati 84018, Italy.
          [3 ] CNISM and Dipartimento di Fisica, Università di Napoli "Federico II", Via Cintia, 26, Naples 80126, Italy. Electronic address: rvelotta@unina.it.
          Article
          S0956-5663(14)00608-3
          10.1016/j.bios.2014.08.020
          25190088
          679c1bfd-6ade-4536-b287-c77ad5cacb73
          History

          Immunosensor,Parathion,Patulin,Photonics immobilization technique,Quartz crystal microbalance

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