Synthesis of a novel monofilament bioabsorbable suture for biomedical applications

Abstract

In this research, a novel bioabsorbable suture that is, monofilament and capable of localized drug delivery, was developed from a combination of natural biopolymers that where not previously applied for this purpose. The optimized suture formulation comprised of sodium alginate (6% wt/vol), pectin (0.1% wt/vol), and gelatin (3% wt/vol), in the presence of glycerol (4% vol/vol) which served as a plasticizer. The monofilament bioabsorbable sutures where synthesized via in situ ionic crosslinking in a barium chloride solution (2% wt/vol). The resulting suture was characterized in terms of mechanical properties, morphology, swelling, degradation, drug release, and biocompatibility, in addition to Fourier‐transform infrared (FTIR) spectroscopy, Powder X‐ray Diffraction (PXRD) and Differential Scanning Calorimetry (DSC) analysis. The drug loaded and non‐drug loaded sutures had a maximum breaking strength of 4.18 and 4.08 N, in the straight configuration and 2.44  N and 2.59  N in the knot configuration, respectively. FTIR spectrum of crosslinked sutures depicted Δ9 cm ⁻¹ downward shift for the carboxyl stretching band which was indicative of ionic interactions between barium ions and sodium alginate. In vitro analysis revealed continued drug release for 7 days and gradual degradation by means of surface erosion, which was completed by day 28. Biocompatibility studies revealed excellent hemocompatibility and no cytotoxicity. These results suggest that the newly developed bioabsorbable suture meets the basic requirements of a suture material and provides a viable alternative to the synthetic polymer sutures that are currently on the market.

Abstract

Graphical representation of the biopolymeric suture fabrication process and characterization thereof.