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      EP1 receptor is involved in prostaglandin E2-induced osteosarcoma growth

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          Abstract

          Recent studies showed that the activation of prostaglandin (PG) receptor EP1 promotes cell migration and invasion in different cancers. The aim of this study was to investigate the role of EP1 in the proliferation of osteosarcoma (OS) cells in vitro and in vivo. EP1 mRNA and protein levels were analyzed by real-time RT-PCR and Western blot, respectively in human OS cell lines MG63, OS732, U-2OS, and 143B compared to human fetal osteoblastic hFOB 1.19 cells. MG63 cells were treated with PGE 2, EP1 specific agonist 17-PT-PGE 2, 17-PT-PGE 2 + EP1 specific antagonist SC51089, or DMSO (control). EP1R-siRNA or a non-silencing irrelevant RNA duplex (negative control) were used for the transfection of MG63 cells, followed by PGE 2 treatment. Nude mice carrying MG63 xenografts were treated with SC51089 (2 mg/kg/day). MG63 cells/xenografts were analyzed by MTT assay, TUNEL assay, PKC enzyme activity assay, and Western blot (EP1 and apoptotic proteins), and tumor growth/volume was evaluated in mice. EP1 levels were significantly higher in OS cells compared to osteoblasts. PGE 2 or 17-PT-PGE 2 treatment increased the proliferation and decreased the apoptosis of MG63 cells. Inhibition of EP1 by SC51089 or siRNA markedly decreased the viability of MG63 cells. Similarly, SC51089 treatment significantly inhibited MG63 cell proliferation and promoted apoptosis in vivo. The silencing of EP1 receptor by siRNA or blockade of EP1 signaling by SC51089 activated extrinsic and intrinsic apoptotic pathways both in vivo and in vitro, as evidenced by increased levels of Bax, cyt c, cleaved caspase-3, caspase-8 and caspase-9. EP1 appears to be involved in PGE 2-induced proliferative activity of MG63 cells. Antagonizing EP1 may provide a novel therapeutic approach to the treatment of OS.

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          Author and article information

          Journal
          Bosn J Basic Med Sci
          Bosn J Basic Med Sci
          Bosnian Journal of Basic Medical Sciences
          Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina (Bosnia )
          1512-8601
          1840-4812
          August 2019
          : 19
          : 3
          : 265-273
          Affiliations
          [1 ]Department of Hand and Foot Surgery, Second Affiliated Hospital of Soochow University, Suzhou, China
          [2 ]Department of Orthopedics, Second Affiliated Hospital of Anhui Medical University, Hefei Anhui, China
          Author notes
          [* ] Corresponding author: Pei-ji Wang, Department of Hand and Foot Surgery, Second Affiliated Hospital of Soochow University, SanXiang Street, No.1055, Suzhou, China. E-mail: wangpeiji88@ 123456163.com
          Article
          PMC6716101 PMC6716101 6716101 BJBMS-19-265
          10.17305/bjbms.2019.4177
          6716101
          30995899
          67eea3a1-e379-48e5-95a9-16fd4ae83cc5
          Copyright: © 2019 ABMSFBIH
          History
          : 03 March 2019
          : 30 March 2019
          Categories
          Research Article

          SC51089,Osteosarcoma,PGE2 ,prostaglandin,EP1
          SC51089, Osteosarcoma, PGE2 , prostaglandin, EP1

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