Avocado Oil Extract Modulates Auditory Hair Cell Function through the Regulation of Amino Acid Biosynthesis Genes

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Abstract

Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.

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Most cited references 33

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Mitochondrial ribosomal RNA mutation associated with both antibiotic-induced and non-syndromic deafness.

T Prezant, J V Agapian, M Bohlman … (1993)

Maternally transmitted non-syndromic deafness was described recently both in pedigrees with susceptibility to aminoglycoside ototoxicity and in a large Arab-Israeli pedigree. Because of the known action of aminoglycosides on bacterial ribosomes, we analysed the sequence of the mitochondrial rRNA genes of three unrelated patients with familial aminoglycoside-induced deafness. We also sequenced the complete mitochondrial genome of the Arab-Israeli pedigree. All four families shared a nucleotide 1555 A to G substitution in the 12S rRNA gene, a site implicated in aminoglycoside activity. Our study offers the first description of a mitochondrial rRNA mutation leading to disease, the first cases of non-syndromic deafness caused by a mitochondrial DNA mutation and the first molecular genetic study of antibiotic-induced ototoxicity.

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Hass Avocado Composition and Potential Health Effects

Mark Dreher, Adrienne J. Davenport (2013)

Hass avocados, the most common commercial avocado cultivars in the world, contain a variety of essential nutrients and important phytochemicals. Although the official avocado serving is one-fifth of a fruit (30 g), according to NHANES analysis the average consumption is one-half an avocado (68 g), which provides a nutrient and phytochemical dense food consisting of the following: dietary fiber (4.6 g), total sugar (0.2 g), potassium (345 mg), sodium (5.5 mg), magnesium (19.5 mg), vitamin A (43 μg), vitamin C (6.0 mg), vitamin E (1.3 mg), vitamin K1 (14 μg), folate (60 mg), vitamin B-6 (0.2 mg), niacin (1.3 mg), pantothenic acid (1.0 mg), riboflavin (0.1 mg), choline (10 mg), lutein/zeaxanthin (185 μg), phytosterols (57 mg), and high-monounsaturated fatty acids (6.7 g) and 114 kcals or 1.7 kcal/g. The avocado oil consists of 71% monounsaturated fatty acids (MUFA), 13% polyunsaturated fatty acids (PUFA), and 16% saturated fatty acids (SFA), which helps to promote healthy blood lipid profiles and enhance the bioavailability of fat soluble vitamins and phytochemicals from the avocado or other fruits and vegetables, naturally low in fat, which are consumed with avocados. There are eight preliminary clinical studies showing that avocado consumption helps support cardiovascular health. Exploratory studies suggest that avocados may support weight management and healthy aging.

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Maternally inherited aminoglycoside-induced and nonsyndromic deafness is associated with the novel C1494T mutation in the mitochondrial 12S rRNA gene in a large Chinese family.

Hui. Zhao, Ronghua Li, Qiuju Wang … (2004)

We report here the characterization of a large Chinese family with maternally transmitted aminoglycoside-induced and nonsyndromic deafness. In the absence of aminoglycosides, some matrilineal relatives in this family exhibited late-onset/progressive deafness, with a wide range of severity and age at onset. Notably, the average age at onset of deafness has changed from 55 years (generation II) to 10 years (generation IV). Clinical data reveal that the administration of aminoglycosides can induce or worsen deafness in matrilineal relatives. The age at the time of drug administration appears to be correlated with the severity of hearing loss experienced by affected individuals. Sequence analysis of mitochondrial DNA in this pedigree identified a homoplasmic C-to-T transition at position 1494 (C1494T) in the 12S rRNA gene. The C1494T mutation is expected to form a novel U1494-1555A base pair, which is in the same position as the C1494-1555G pair created by the deafness-associated A1555G mutation, at the highly conserved A site of 12S rRNA. Exposure to a high concentration of paromomycin or neomycin caused a variable but significant average increase in doubling time in lymphoblastoid cell lines derived from four symptomatic and two asymptomatic individuals in this family carrying the C1494T mutation when compared to four control cell lines. Furthermore, a significant decrease in the rate of total oxygen consumption was observed in the mutant cell lines. Thus, our data strongly support the idea that the A site of mitochondrial 12S rRNA is the primary target for aminoglycoside-induced deafness. These results also strongly suggest that the nuclear background plays a role in the aminoglycoside ototoxicity and in the development of the deafness phenotype associated with the C1494T mutation in the mitochondrial 12S rRNA gene.

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Journal

Journal ID (nlm-ta): Nutrients

Journal ID (iso-abbrev): Nutrients

Journal ID (publisher-id): nutrients

Title: Nutrients

Publisher: MDPI

ISSN (Electronic): 2072-6643

Publication date (Electronic): 08 January 2019

Publication date Collection: January 2019

Volume: 11

Issue: 1

Electronic Location Identifier: 113

Affiliations

[1 ]Department of Oriental Medicine Biotechnology, College of Life Sciences and Graduate School of Biotechnology, Kyung Hee University, Global Campus, Gyeonggi 17104, Korea; 01030084217@ 123456hanmail.net (Y.H.N.); isabelula3r@ 123456gmail.com (I.R.); wanlapa.nuankaew@ 123456gmail.com (W.N.); caty05@ 123456naver.com (Y.H.K.); rodrigocastanedaqf@ 123456gmail.com (R.C.); tjdbf26@ 123456gmail.com (S.Y.J.); 01026793977@ 123456hanmail.net (M.S.P.); habina22@ 123456hanmail.net (B.N.H.)

[2 ]Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon 21936, Korea; syjeong46@ 123456naver.com (S.Y.J.); phamnguyenminhthud12@ 123456gmail.com (T.N.M.P.)

[3 ]R&D Center, Dongkook Pharm. Co., Ltd., Gyeonggi 16229, Korea; lkw1@ 123456dkpharm.co.kr (K.W.L.); ljs@ 123456dkpharm.co.kr (J.S.L.); Kdh2@ 123456dkpharm.co.kr (D.H.K.); pyh@ 123456dkpharm.co.kr (Y.H.P.)

[4 ]College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 21983, Korea; kimsh11@ 123456yonsei.ac.kr

[5 ]Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul 03722, Korea; ISMOONMD@ 123456yuhs.ac

[6 ]Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Seoul 02453, Korea; sychoung@ 123456khu.ac.kr

Author notes

[* ]Correspondence: kwjeong@ 123456gachon.ac.kr (K.W.J.); panjae@ 123456khu.ac.kr (T.H.K.); Tel.: +82-31-201-3862 (T.H.K.); +82-32-820-4925 (K.W.J.); Fax: +82-303-0300-0030 (T.H.K.); +82-32-820-4829 (K.W.J.)

[†]

These two authors contributed equally to this work.

Author information

Rodrigo Castañeda https://orcid.org/0000-0003-4938-9179

Kwang Won Jeong https://orcid.org/0000-0003-2258-8354

Tong Ho Kang https://orcid.org/0000-0002-4161-6798

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Publisher ID: nutrients-11-00113

DOI: 10.3390/nu11010113

PMC ID: 6356572

PubMed ID: 30626089

SO-VID: 681a4516-6a76-4076-8d72-58b2586d5673

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

Avocado Oil Extract Modulates Auditory Hair Cell Function through the Regulation of Amino Acid Biosynthesis Genes

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Karger: Nutrition and Dietetics

Most cited references 33

Mitochondrial ribosomal RNA mutation associated with both antibiotic-induced and non-syndromic deafness.

Hass Avocado Composition and Potential Health Effects

Maternally inherited aminoglycoside-induced and nonsyndromic deafness is associated with the novel C1494T mutation in the mitochondrial 12S rRNA gene in a large Chinese family.

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