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      Chromatographic methods for the isolation, separation and characterisation of dissolved organic matter

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          Abstract

          This review presents an overview of the separation techniques applied to the complex challenge of dissolved organic matter characterisation.

          Abstract

          This review presents an overview of the separation techniques applied to the complex challenge of dissolved organic matter characterisation. The review discusses methods for isolation of dissolved organic matter from natural waters, and the range of separation techniques used to further fractionate this complex material. The review covers both liquid and gas chromatographic techniques, in their various modes, and electrophoretic based approaches. For each, the challenges that the separation and fractionation of such an immensely complex sample poses is critically reviewed.

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          Evaluation of specific ultraviolet absorbance as an indicator of the chemical composition and reactivity of dissolved organic carbon.

          Specific UV absorbance (SUVA) is defined as the UV absorbance of a water sample at a given wavelength normalized for dissolved organic carbon (DOC) concentration. Our data indicate that SUVA, determined at 254 nm, is strongly correlated with percent aromaticity as determined by 13C NMR for 13 organic matter isolates obtained from a variety of aquatic environments. SUVA, therefore, is shown to be a useful parameter for estimating the dissolved aromatic carbon content in aquatic systems. Experiments involving the reactivity of DOC with chlorine and tetramethylammonium hydroxide (TMAH), however, show a wide range of reactivity for samples with similar SUVA values. These results indicate that, while SUVA measurements are good predictors of general chemical characteristics of DOC, they do not provide information about reactivity of DOC derived from different types of source materials. Sample pH, nitrate, and iron were found to influence SUVA measurements.
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            Spectrofluorometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity

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              Absorption by dissolved organic matter of the sea (yellow substance) in the UV and visible domains1

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                Author and article information

                Journal
                ESPICZ
                Environmental Science: Processes & Impacts
                Environ. Sci.: Processes Impacts
                Royal Society of Chemistry (RSC)
                2050-7887
                2050-7895
                2015
                2015
                : 17
                : 9
                : 1531-1567
                Affiliations
                [1 ]Australian Centre for Research on Separation Sciences (ACROSS)
                [2 ]University of Tasmania
                [3 ]Hobart
                [4 ]Australia 7001
                [5 ]Central Science Laboratory (CSL)
                [6 ]Irish Separation Science Cluster
                [7 ]National Centre for Sensor Research
                [8 ]School of Chemical Sciences
                [9 ]Dublin City University
                [10 ]Dublin 9
                Article
                10.1039/C5EM00223K
                26290053
                6823e076-a4f4-467e-9222-0fddcb696442
                © 2015
                History

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