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      Sensibilidade da técnica de reação em cadeia da polimerase para HIV-1 em relação à técnica de ensaio imunoenzimático Translated title: Polymerase chain reaction sensitivity against enzyme immuno assay test virus HIV -1

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          Abstract

          O vírus da imunodeficiência humana (HIV) é o agente etiológico da Síndrome da Imunodeficiência Adquirida, AIDS, uma doença de grande preocupação médica. O genoma deste vírus encontra-se arranjado em nove genes individuais e por duas estruturas idênticas denominadas de repetições terminais nas extremidades 5' e 3'. Tivemos como objetivo analisar a sensibilidade do teste da reação em cadeia da polimerase (PCR) e o teste de ensaio imunoenzimático (ELISA), como teste para triagem de doadores de sangue para HIV. Foram analisadas 200 amostras de doadores e pacientes, da Fundação HEMOPA, com padrão positivo e indeterminado no teste ELISA. Na triagem sorológica pelo ELISA tiveram como resultado 35 amostras positivas, 75 amostras negativas e 90 amostras indeterminadas as quais foram submetidas ao teste pela PCR. Vinte e cinco amostras tiveram resultado positivo e 175 amostras negativas. Com estes resultados concluímos que a reação de PCR apresenta-se positiva somente nas amostras em que o teste ELISA apresenta relação DO/cutoff, acima de 3.

          Translated abstract

          The Human Immunodeficiency Virus (HIV) is the etiological agent of the Acquired Immunodeficiency Syndrome, (AIDS), a disease of great concern in the medical world. The genome of this virus is found arranged in nine genes and by two identical structures named terminal repetitions on the 5' and 3' extremities. As our objective, the sensitivity of the Polymerase Chain Reaction (PCR) and Enzyme-Linked Immunoabsorbent Assay (ELISA) tests were analyzed as an evaluation for blood donors who are infected with HIV. Two hundred samples from donors and patients from the HEMOPA Foundation were analyzed, with the positive and indeterminate standard of the ELISA test. In the ELISA test selection, the results were thirty-five positive samples, seventy-five negative samples and ninety indeterminate samples. These samples were subsequently submitted to the PCR test where twenty-five samples had a positive result and one hundred seventy five had negative results. With these results we conclude that only samples that had a relation between the optical density and the cutoff above three (ELISA) were found positive in the PCR test.

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          Polymerase chain reaction.

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            Optimal Conditions for Detection of Human Immunodeficiency Virus Type 1 DNA by Polymerase Chain Reaction with Nested Primers

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              The Retroviridae

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rbhh
                Revista Brasileira de Hematologia e Hemoterapia
                Rev. Bras. Hematol. Hemoter.
                Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (São Paulo )
                1806-0870
                March 2002
                : 24
                : 1
                : 25-28
                Affiliations
                [1 ] Fundação Hemopa Brazil
                [2 ] Fundação Hemopa Brazil
                [3 ] Universidade Federal do Pará Brazil
                Article
                S1516-84842002000100005
                10.1590/S1516-84842002000100005
                685325ca-7dd8-4b22-874d-65b2e76b6793

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1516-8484&lng=en
                Categories
                HEMATOLOGY
                MEDICINE, RESEARCH & EXPERIMENTAL

                Medicine,Hematology
                HIV-1,PCR,ELISA
                Medicine, Hematology
                HIV-1, PCR, ELISA

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