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      Diallelic analysis for lysine and oil contents in maize grains Translated title: Análise dialélica para conteúdos de lisina e óleo em grãos de milho

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          Abstract

          Six S5 lines of maize, with differences for lysine and oil contents in grains, were used to carry out a complete series of diallelic crosses. The resulting 15 hybrids were grown in a field at two nitrogen levels (10 and 130 kg N ha-1). The general combining ability (GCA) and specific combining ability (SCA) were obtained by using the method 4, model I of Griffing for grain yield, and grain lysine and oil contents. Significant (p < 0.001) interaction was observed between GCA and N levels for grain yield, indicating the selection of different lines for each N level. This interaction was not significant for lysine content, but there were significant effects of GCA for this trait at both N levels (p < 0.1). Significant effects were not observed for GCA or SCA for oil content, however a positive correlation was observed between lysine and oil contents in the hybrids, in the lines and even in the control cultivars. The results indicate the effectiveness of selection for lysine content, irrespective of N level, in the studied non-opaque maize lines, and the possibility of achieving both high lysine and oil content in grains.

          Translated abstract

          Seis linhagens S5 de milho, com diferenças para conteúdos de óleo e de lisina nos grãos, foram usadas para realizar uma série completa de cruzamentos dialélicos. Os 15 híbridos resultantes foram cultivados em campo com dois níveis de nitrogênio (10 e 130 kg N ha-1). A capacidade geral de combinação (CGC) e a capacidade específica de combinação (CEC) foram obtidas com o método 4, modelo I de Griffing para produtividade de grãos e concentração de lisina e óleo nos grãos. Interação significativa (p < 0.001) foi observada entre CGC e níveis de N para produtividade de grãos, indicando seleção de diferentes linhagens para cada nível de N. Esta interação não foi significativa para conteúdo de lisina, mas existiu efeito significativo (p < 0.1) da CGC nos dois níveis de N para esta variável. Os efeitos de CGC e CEC foram não significativos para conteúdo de óleo, contudo, foi observada correlação positiva entre conteúdos de lisina e óleo nos híbridos, nas linhagens e mesmo nas cultivares utilizadas como controle. Os resultados indicam a efetividade da seleção para conteúdo de lisina nas linhagens não opaco de milho estudadas, independente dos níveis de N, e a possibilidade de atingir concomitantemente maiores níveis de óleo e lisina nos grãos.

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          Most cited references64

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          Genética biométrica no fitomelhoramento.

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            Concept of General and Specific Combining Ability in Relation to Diallel Crossing Systems

            B Griffing (1956)
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              The accumulation of oleosins determines the size of seed oilbodies in Arabidopsis.

              We investigated the role of the oilbody proteins in developing and germinating Arabidopsis thaliana seeds. Seed oilbodies are simple organelles comprising a matrix of triacylglycerol surrounded by a phospholipid monolayer embedded and covered with unique proteins called oleosins. Indirect observations have suggested that oleosins maintain oilbodies as small single units preventing their coalescence during seed desiccation. To understand the role of oleosins during seed development or germination, we created lines of Arabidopsis in which a major oleosin is ablated or severely attenuated. This was achieved using RNA interference techniques and through the use of a T-DNA insertional event, which appears to interrupt the major (18 kD) seed oleosin gene of Arabidopsis and results in ablation of expression. Oleosin suppression resulted in an aberrant phenotype of embryo cells that contain unusually large oilbodies that are not normally observed in seeds. Changes in the size of oilbodies caused disruption of storage organelles, altering accumulation of lipids and proteins and causing delay in germination. The aberrant phenotypes were reversed by reintroducing a recombinant oleosin. Based on this direct evidence, we have shown that oleosins are important proteins in seed tissue for controlling oilbody structure and lipid accumulation.
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                Author and article information

                Journal
                sa
                Scientia Agricola
                Sci. agric. (Piracicaba, Braz.)
                São Paulo - Escola Superior de Agricultura "Luiz de Queiroz" (Piracicaba, SP, Brazil )
                1678-992X
                April 2009
                : 66
                : 2
                : 204-209
                Affiliations
                [01] Seropédica RJ orgnameUFRRJ orgdiv1Depto. de Ciências Fisiológicas Brasil
                [03] Piracicaba SP orgnameUSP orgdiv1CENA orgdiv2Lab. de Melhoramento de Plantas Brasil
                [02] Piracicaba SP orgnameUSP orgdiv1ESALQ orgdiv2Depto. de Genética Brasil
                Article
                S0103-90162009000200009 S0103-9016(09)06600209
                10.1590/S0103-90162009000200009
                685b63c6-150a-4c71-ae69-002013767dad

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 28 July 2008
                : 19 October 2007
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 39, Pages: 6
                Product

                SciELO Brazil

                Categories
                Genetics and Plant Breeding

                proteínas,cereal,opaque,infravermelho próximo,nitrogen,proteins,near infrared,opaco,nitrogênio

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