Transcriptomic Response of Nannochloropsis oceanica to Benzo[a]pyrene

Abstract

In this study, the RNA sequencing was used to describe the response of Nannochloropsis oceanica, a marine microalga, to benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, in order to elucidate the metabolic pathways (or processes) involved in microalgal response to this stubborn pollutant. N. oceanica was exposed to BaP at a concentration of 90 µg L ⁻¹ for 72 h, and its transcriptome was sequenced through the Illumina HiSeq™ 2500 platform. This concentration of BaP was selected as it is the lowest for modeling the most appropriate growth inhibition of N. oceanica for transcriptomic analysis. We found that N. oceanica responds to BaP through degrading proteins and repairing DNA damaged by BaP. In addition, superoxide dismutase (SOD) strengthened its performance by increasing its transcript abundance. The physiological mechanism underlining the response of N. oceanica to BaP as revealed by transcriptomic analysis was consistent with the biochemical insights documented previously.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 14 May 2019

Publication date (Print): 01 June 2019

Volume: 18

Issue: 3

Pages: 680-686

Affiliations

¹ Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China

² College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

³ Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China

Author notes

*Corresponding author: GUO Li; YANG Guanpin

Article

Publisher ID: s11802-019-3830-9

DOI: 10.1007/s11802-019-3830-9

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

License:

The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

Product

Self URI (journal-page): https://www.springer.com/journal/11802