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      • Record: found
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      • Article: found

      Species Variation of Some Acid Hydrolases in the Normal Cornea

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          Abstract

          Acid phosphatase, N-acetyl- β-D-glucosaminidase, β-alactosidase and β-glucuronidase were investigated in the normal corneas of several animals (rabbit, calf, bovine, dog, pig, and sheep) by means of biochemical and histochemical methods. The results of both approaches show a wide interspecies variety which are, in general, in good agreement. Pig and sheep corneas show lower activities of all enzymes investigated than those of other animals. The best correlation was found in the case of acid phosphatase which is highest in all animal species under investigation, and is most active in the epithelium as could be proved histochemically. On the other hand, acid glycosidases are more pronounced in keratocytes than in epithelial and endothelial cells. Sequence of activities of individual acid glycosidases, determined by quantitative analysis and histochemical methods, is discussed.

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          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1977
          Publication date (Print): 1977
          Publication date (Electronic): 03 December 2009
          Volume: 9
          Issue: 3
          Pages: 155-161
          Affiliations
          Institute of Experimental Medicine (Director: Doc. Dr. V. Kusák, DrSc) Czechoslovak Academy of Sciences, Prague
          Article
          Publisher ID: 264880 Other ID: Ophthalmic Res 1977;9:155–161
          DOI: 10.1159/000264880
          SO-VID: 68ec6fbd-f5c9-46ab-ac70-ef092638d6db
          Copyright © © 1977 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 28 March 1977
          Date accepted : 02 June 1977
          Page count
          Pages: 7
          Categories
          Subject: Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: Acid phosphatase,Cornea of various species,Histochemical demonstration,Biochemical data,N-acetyl-<italic>β</italic>-glucosaminidase,<italic>β</italic>-Galactosidase,<italic>β</italic>-Glucuronidase
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: Acid phosphatase, Cornea of various species, Histochemical demonstration, Biochemical data, N-acetyl-<italic>β</italic>-glucosaminidase, <italic>β</italic>-Galactosidase, <italic>β</italic>-Glucuronidase

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