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      Yeast DNA polymerase epsilon participates in leading-strand DNA replication.

      Science (New York, N.Y.)

      Base Pair Mismatch, DNA Polymerase II, genetics, metabolism, DNA Replication, DNA, Fungal, Fungal Proteins, Mutation, Point Mutation, Replication Origin, Saccharomyces cerevisiae, enzymology

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          Abstract

          Multiple DNA polymerases participate in replicating the leading and lagging strands of the eukaryotic nuclear genome. Although 50 years have passed since the first DNA polymerase was discovered, the identity of the major polymerase used for leading-strand replication is uncertain. We constructed a derivative of yeast DNA polymerase epsilon that retains high replication activity but has strongly reduced replication fidelity, particularly for thymine-deoxythymidine 5'-monophosphate (T-dTMP) but not adenine-deoxyadenosine 5'-monophosphate (A-dAMP) mismatches. Yeast strains with this DNA polymerase epsilon allele have elevated rates of T to A substitution mutations. The position and rate of these substitutions depend on the orientation of the mutational reporter and its location relative to origins of DNA replication and reveal a pattern indicating that DNA polymerase epsilon participates in leading-strand DNA replication.

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          Author and article information

          Journal
          17615360
          2233713
          10.1126/science.1144067

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