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      Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol.

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          Abstract

          A novel aptamer-based label-free electrochemical impedance spectroscopy biosensor for 17β-estradiol has been fabricated. The aptamers were firstly immobilized on the gold electrode through Au-S interaction; the aptamer probe was then bound with the addition of 17β-estradiol to form the estradiol/aptamer complex on the electrode surface. This leads to a significantly larger interfacial electron transfer resistance than that without the addition of 17β-estradiol. The change in the resistance had a linear relationship with 17β-estradiol concentration in the range of 1.0 × 10(-8) to 1.0 × 10(-11) mol L(-1), with a detection limit of 2.0 × 10(-12) mol L(-1). The biosensor showed high selectivity to 17β-estradiol and good stability. The designed biosensor has been applied to detect 17β-estradiol in human urine with satisfactory results.

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          Author and article information

          Journal
          Analyst
          The Analyst
          1364-5528
          0003-2654
          Feb 21 2012
          : 137
          : 4
          Affiliations
          [1 ] Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zylin@fzu.edu.cn
          Article
          10.1039/c1an15856b
          22158706
          69171334-e467-47a5-a608-9a5a4e0c3202
          History

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