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      Draft Whole-Genome Sequence of Triparma laevis f. inornata (Parmales, Bolidophyceae), Isolated from the Oyashio Region, Western North Pacific Ocean

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          Abstract

          We present the first draft whole-genome sequence for the Parmales (Bolidophyceae, Heterokonta), a picoplanktonic sister group of diatoms, using a Triparma laevis f. inornata strain that was isolated from the Oyashio region in the western North Pacific Ocean.

          ABSTRACT

          We present the first draft whole-genome sequence for the Parmales (Bolidophyceae, Heterokonta), a picoplanktonic sister group of diatoms, using a Triparma laevis f. inornata strain that was isolated from the Oyashio region in the western North Pacific Ocean.

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          Toward almost closed genomes with GapFiller

          De novo assembly is a commonly used application of next-generation sequencing experiments. The ultimate goal is to puzzle millions of reads into one complete genome, although draft assemblies usually result in a number of gapped scaffold sequences. In this paper we propose an automated strategy, called GapFiller, to reliably close gaps within scaffolds using paired reads. The method shows good results on both bacterial and eukaryotic datasets, allowing only few errors. As a consequence, the amount of additional wetlab work needed to close a genome is drastically reduced. The software is available at http://www.baseclear.com/bioinformatics-tools/.
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            Visualizing data using ti-SNE

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              Sequencing and analysis of the complete organellar genomes of Parmales, a closely related group to Bacillariophyta (diatoms)

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                Author and article information

                Contributors
                Role: Editor
                Journal
                Microbiol Resour Announc
                Microbiol Resour Announc
                ga
                mra
                MRA
                Microbiology Resource Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2576-098X
                13 August 2020
                August 2020
                : 9
                : 33
                : e00367-20
                Affiliations
                [a ]Tohoku National Fisheries Research Institute, Fisheries Research and Education Agency, Shiogama, Miyagi, Japan
                [b ]National Research Institute of Fisheries Science, Fisheries Research and Education Agency, Yokohama, Kanagawa, Japan
                [c ]Prefectural University of Kumamoto, Kumamoto, Kumamoto, Japan
                [d ]University of Tokyo, Tokyo, Japan
                University of California, Riverside
                Author notes
                Address correspondence to Akira Kuwata, akuwata@ 123456affrc.go.jp .

                Author order was determined on the basis of affiliation and seniority.

                Citation Kuwata A, Saitoh K, Nakamura Y, Ichinomiya M, Sato N. 2020. Draft whole-genome sequence of Triparma laevis f. inornata (Parmales, Bolidophyceae), isolated from the Oyashio region, western North Pacific Ocean. Microbiol Resour Announc 9:e00367-20. https://doi.org/10.1128/MRA.00367-20.

                Author information
                https://orcid.org/0000-0002-7359-7421
                https://orcid.org/0000-0001-6230-0410
                Article
                MRA00367-20
                10.1128/MRA.00367-20
                7427184
                32817146
                695377a5-d9e9-43c4-9e76-7c2f2b22f952
                Copyright © 2020 Kuwata et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                : 17 April 2020
                : 14 July 2020
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 11, Pages: 2, Words: 1416
                Funding
                Funded by: The Canon Foundation (Japan);
                Award Recipient :
                Funded by: MEXT | Japan Society for the Promotion of Science (JSPS), https://doi.org/10.13039/501100001691;
                Award ID: 22657027
                Award ID: 23370046
                Award ID: 26291085
                Award ID: 17H03724
                Award Recipient :
                Categories
                Genome Sequences
                Custom metadata
                August 2020

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