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      Antagonistic process of Dicyma pulvinata against Fusicladium macrosporum on rubber tree Translated title: Processo de antagonismo de Dicyma pulvinata contra Fusicladium macrosporum em folhas de seringueira

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          Abstract

          The interaction between Dicyma pulvinata and Fusicladium macrosporum was studied by scanning electron microscopy. Spores of D. pulvinata germinated on the surface of F. macrosporum lesions induced on artificially infected rubber plants were fixed 8 h after inoculation. D. pulvinata germ tubes seemed to elongate toward F. macrosporum. Close contact between the antagonistic fungus and F. macrosporum spores was verified 24 h after application of D. pulvinata. At the end of the process, spores of F. macrosporum seemed to have disintegrated and to be devoid of content. The hyperparasite grew completely over the pathogen. Six to seven days after application of the antagonistic fungus, D. pulvinata conidiophores were observed emerging from F. macrosporum structures with profuse sporulation. Studies have also shown the possibility of D. pulvinata producing hydrolytic enzymes, which could be associated with the control of plant pathogens. This information may help to elucidate some of the modes of action of D. pulvinata, a potential biological control agent for South American leaf blight of Hevea rubber plant.

          Translated abstract

          Estudou-se a interação entre Dicyma pulvinata e F. macrosporum ao microscópio eletrônico de varredura. Esporos de D. pulvinata germinaram na superfície das lesões induzidas por F. macrosporum em plantas de seringueira (Hevea brasiliensis), infectadas artificialmente, fixadas 8 h após a inoculação do antagonista. Aparentemente, os tubos germinativos se alongaram em direção ao patógeno. O contato íntimo entre o hiperparasita e o patógeno foi verificado em amostras fixadas 24 h após a aplicação de D. pulvinata. Ao término do processo, os esporos de F. macrosporum aparentemente invadidos pelo antagonista mostraram-se desintegrados e esvaziados de seu conteúdo. D. pulvinata cresceu sobre as lesões, sobrepondo totalmente o patógeno. Seis dias após a aplicação, conidióforos do fungo antagonista foram observados emergindo das estruturas do patógeno, produzindo esporos em grande quantidade. Verificou-se, também, um possível envolvimento de enzimas hidrolíticas na associação antagonística entre D. pulvinata e o patógeno. Estas informações podem contribuir para elucidar o modo de ação de D. pulvinata, um potencial agente de controle biológico para o mal das folhas da seringueira.

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          Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

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            A simplification of the protein assay method of Lowry et al. which is more generally applicable.

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              Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum

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                Author and article information

                Journal
                tpp
                Tropical Plant Pathology
                Trop. plant pathol.
                Sociedade Brasileira de Fitopatologia (Brasília, DF, Brazil )
                1982-5676
                1983-2052
                February 2008
                : 33
                : 1
                : 5-11
                Affiliations
                [01] Brasília DF orgnameEmbrapa Recursos Genéticos e Biotecnologia Brazil
                [02] Brasília DF orgnameUniversidade de Brasília orgdiv1Departamento de Fitopatologia Brazil
                [03] Brasília DF orgnameCentro Universitário de Brasília Brazil
                Article
                S1982-56762008000100002 S1982-5676(08)03300102
                10.1590/S1982-56762008000100002
                697a6f24-539f-49ce-b509-16ac6db68b14

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 25 February 2008
                : 03 September 2007
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 29, Pages: 7
                Product

                SciELO Brazil

                Categories
                Articles

                South American Leaf Blight,pathogen and antagonistic interaction,mal-das-folhas da seringueira,Hevea brasiliensis,biocontrole,interação patógeno e antagonista,biocontrol

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