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      CNS Distribution of Members of the Two-Pore-Domain (KCNK) Potassium Channel Family

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          Abstract

          Two-pore-domain potassium (K +) channels are substrates for resting K + currents in neurons. They are major targets for endogenous modulators, as well as for clinically important compounds such as volatile anesthetics. In the current study, we report on the CNS distribution in the rat and mouse of mRNA encoding seven two-pore-domain K + channel family members: TASK-1 (KCNK3), TASK-2 (KCNK5), TASK-3 (KCNK9), TREK-1 (KCNK2), TREK-2 (KCNK10), TRAAK (KCNK4), and TWIK-1 (KCNK1). All of these genes were expressed in dorsal root ganglia, and for all of the genes except TASK-2, there was a differential distribution in the CNS. For TASK-1, highest mRNA accumulation was seen in the cerebellum and somatic motoneurons. TASK-3 was much more widely distributed, with robust expression in all brain regions, with particularly high expression in somatic motoneurons, cerebellar granule neurons, the locus ceruleus, and raphe nuclei and in various nuclei of the hypothalamus. TREK-1 was highest in the striatum and in parts of the cortex (layer IV) and hippocampus (CA2 pyramidal neurons). mRNA for TRAAK also was highest in the cortex, whereas expression of TREK-2 was primarily restricted to the cerebellar granule cell layer. There was widespread distribution of TWIK-1, with highest levels in the cerebellar granule cell layer, thalamic reticular nucleus, and piriform cortex. The differential expression of each of these genes likely contributes to characteristic excitability properties in distinct populations of neurons, as well as to diversity in their susceptibility to modulation.

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          Author and article information

          Journal
          J Neurosci
          J. Neurosci
          jneuro
          jneurosci
          J. Neurosci
          The Journal of Neuroscience
          Society for Neuroscience
          0270-6474
          1529-2401
          1 October 2001
          : 21
          : 19
          : 7491-7505
          Affiliations
          [ 1 ]Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, and
          [ 2 ]Department of Physiology and Biophysics, Finch University of Health Sciences, The Chicago Medical School, North Chicago, Illinois 60064
          Article
          PMC6762917 PMC6762917 6762917 5701
          10.1523/JNEUROSCI.21-19-07491.2001
          6762917
          11567039
          69dddcab-91e5-4732-972f-32780aa09cc9
          Copyright © 2001 Society for Neuroscience
          History
          : 3 May 2001
          : 3 July 2001
          : 26 July 2001
          Categories
          ARTICLE
          Cellular/Molecular
          Custom metadata
          5.00

          TASK,KCNK, in situ hybridization,potassium channel,TREK,TRAAK,TWIK
          TASK, KCNK, in situ hybridization, potassium channel, TREK, TRAAK, TWIK

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