10
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants

      ,
      Biochimica et Biophysica Acta (BBA) - Biomembranes
      Elsevier BV

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          <p class="first" id="d9244576e51">Chinese hamster ovary cells selected for resistance to colchicine display pleiotropic cross-resistance to a wide range of amphiphilic drugs. The drug-resistant phenotype is due to a membrane alteration which reduces the rate of drug permeation. Surface labelling studies reveal that drug-resistant Chinese hamster ovary cell membranes possess a carbohydrate-containing component of 170 000 daltons apparent molecular weight which is not observed in wild type cells. Through studies of the metabolic incorporation of carbohydrate and protein precursors, and through the use of selective proteolysis, this component is shown to be a cell surface glycoprotein. Since this glycoprotein appears unique to mutant cells displaying altered drug permeability, we have designated it the P glycoprotein. The relative amount of surface labelled P glycoprotein correlates with the degree of drug resistance in a number of independent mutant and revertant clones. A similar high molecular weight glycoprotein is also present in drug-resistant mutants from another hamster cell line. Observations on the molecular basis of pleiotropic drug resistance are interpreted in terms of a model wherein certain surface glycoproteins control drug permeation by modulating the properties of hydrophobic membrane regions... </p>

          Related collections

          Author and article information

          Journal
          Biochimica et Biophysica Acta (BBA) - Biomembranes
          Biochimica et Biophysica Acta (BBA) - Biomembranes
          Elsevier BV
          00052736
          November 1976
          November 1976
          : 455
          : 1
          : 152-162
          Article
          10.1016/0005-2736(76)90160-7
          990323
          6a38ba28-4a60-46ab-bc7f-0075c3f74877
          © 1976

          http://www.elsevier.com/tdm/userlicense/1.0/

          History

          Comments

          Comment on this article