For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
40
views
28
references
 Top references
cited by
9
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      37
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      The differential activation of intracellular signaling pathways confers the permissiveness of embryonic stem cell derivation from different mouse strains

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The requirement of leukemia inhibitory factor (LIF) for the establishment and maintenance of mouse embryonic stem cells (ESCs) depends on the genetic background of the ESC origin. To reveal the molecular basis of the strain-dependent function of LIF, we compared the activation of the intracellular signaling pathways downstream of LIF in ESCs with different genetic backgrounds. We found that the JAK-Stat3 pathway was dominantly activated in ESCs derived from ‘permissive’ mouse strains (129Sv and C57BL6), whereas the MAP kinase pathway was hyperactivated in ESCs from ‘non-permissive’ strains (NOD, CBA and FVB). Artificial activation of Stat3 supported stable self-renewal of ESCs from non-permissive strains. These data suggest that the difference in the balance between the two intracellular signaling pathways underlies the differential response to LIF.

          Abstract

          Highlighted article: Different mouse strains show distinct JAK-Stat and MAPK activity responses to LIF treatment, accounting for the varying ease of generating stem cells from these lines.

          Related collections

          Most cited references28

          • Record: found
          • Abstract: found
          • Article: not found

          Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.

          G Martin (1981)
          This report describes the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice. The pluripotency of these embryonic stem cells was demonstrated conclusively by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types. Such embryonic stem cells were isolated from inner cell masses of late blastocysts cultured in medium conditioned by an established teratocarcinoma stem cell line. This suggests that such conditioned medium might contain a growth factor that stimulates the proliferation or inhibits the differentiation of normal pluripotent embryonic cells, or both. This method of obtaining embryonic stem cells makes feasible the isolation of pluripotent cells lines from various types of noninbred embryo, including those carrying mutant genes. The availability of such cell lines should made possible new approaches to the study of early mammalian development.
          Article 0 comments Cited 712 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Establishment in culture of pluripotential cells from mouse embryos.

            Sydney M. Evans, H Kaufman (1981)
            Article 0 comments Cited 674 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Stat3 as an oncogene.

              J. Bromberg, M Wrzeszczynska, G Devgan … (1999)
              STATs are latent transcription factors that mediate cytokine- and growth factor-directed transcription. In many human cancers and transformed cell lines, Stat3 is persistently activated, and in cell culture, active Stat3 is either required for transformation, enhances transformation, or blocks apoptosis. We report that substitution of two cysteine residues within the C-terminal loop of the SH2 domain of Stat3 produces a molecule that dimerizes spontaneously, binds to DNA, and activates transcription. The Stat3-C molecule in immortalized fibroblasts causes cellular transformation scored by colony formation in soft agar and tumor formation in nude mice. Thus, the activated Stat3 molecule by itself can mediate cellular transformation and the experiments focus attention on the importance of constitutive Stat3 activation in human tumors.
              Article 0 comments Cited 393 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Development
                Journal ID (iso-abbrev): Development
                Journal ID (publisher-id): DEV
                Journal ID (hwp): develop
                Title: Development (Cambridge, England)
                Publisher: The Company of Biologists
                ISSN (Print): 0950-1991
                ISSN (Electronic): 1477-9129
                Publication date (Print): 1 February 2015
                Publication date PMC-release: 1 February 2015
                Volume: 142
                Issue: 3
                Pages: 431-437
                Affiliations
                [1 ]Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental Biology (CDB) , Minatojima-minamimachi 2-2-3, Chuo-Ku, Kobe 650-0047, Japan
                [2 ]CREST (Core Research for Evolutional Science and Technology), Japan Science Technology Agency , Honcho 4-1-8, Kawaguchi, Saitama 332-0012, Japan
                [3 ]Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine , 7-5-1 Kusunokicho, Chuo-ku, Kobe 6500017, Japan
                Author notes
                [* ]Author for correspondence ( niwa@ 123456cdb.riken.jp )
                Article
                Publisher ID: DEV112375
                DOI: 10.1242/dev.112375
                PMC ID: 4302992
                PubMed ID: 25564647
                SO-VID: 6a4521ff-7b0c-4024-84da-ddac56d4847a
                Copyright © © 2015. Published by The Company of Biologists Ltd
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

                History
                Date received : 6 May 2014
                Date accepted : 5 December 2014
                Categories
                Subject: 102
                Subject: Stem Cells and Regeneration

                ScienceOpen disciplines: Developmental biology
                Keywords: lif signaling,map kinase,stat3,embryonic stem cell,signal responsiveness
                Data availability:
                ScienceOpen disciplines: Developmental biology
                Keywords: lif signaling, map kinase, stat3, embryonic stem cell, signal responsiveness

                Comments

                Comment on this article

                scite_

                Similar content37

                See all similar

                Cited by9

                See all cited by

                Most referenced authors1,106

                See all reference authors