Preparative isolation and purification of calycosin from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography
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Abstract
Calycosin was purified from an ethyl acetate extract of the root of Astragalus membranaceus
Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography. The
separation was performed in two steps with a two-phase solvent system composed of
n-hexane-chloroform-methanol-water (1:3:3:2, v/v). From 200 mg of the crude extract,
14.8 mg of calycosin was obtained at over 99% purity as determined by HPLC analysis,
and its chemical structure was confirmed by MS, 1H and 13C nuclear magnetic resonance.