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      Comportamiento reproductivo de ovejas F1 (Damara x Merino) sincronizadas con CIDR y dos tiempos de aplicación de GnRH Translated title: Reproductive performance of F1 (Damara x Merino) ewes synchronised with CIDR and two times of GnRH injection

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          Abstract

          Este experimento se realizó para evaluar el comportamiento reproductivo en ovejas F1 (Damara x Merino) importadas de Australia, sincronizadas con un dispositivo liberador de la hormona (CIDR) y dos tiempos de aplicación de hormona liberadora de gonadotropinas (GnRH). Cuarenta y cinco ovejas F1 de primer parto con 18.08 ± 0.07 meses de edad y 43.3 ± 5.6 kg de peso corporal, fueron asignadas aleatoriamente a uno de tres tratamientos: T1 (n = 15): CIDR por doce días; T2 (n = 15): CIDR por doce días mas 25 μg de GnRH 48 h antes del retiro del dispositivo y T3 (n = 15): CIDR por doce días mas 25 μg de GnRH al momento del retiro del CIDR. El porcentaje de presentación de estros (100%) fue similar (p > 0.05) para T1, T2 y T3. El intervalo (h) de inicio del estro sincronizado después del retiro del CIDR fue diferente (p <0.05) en T2 y T3 (43.86 ± 5.42 y 45.73 ± 3.36 h) comparado con T1 (T1 = 37.33 ± 7.90 h). El porcentaje de fertilidad a estro sincronizado fue similar (p > 0.05) para T1, T2 y T3 (60, 53.3 y 46.6%, respectivamente). La prolificidad promedio general fue de 100.8% y fue similar (p > 0.05) entre tratamientos. La concentración de progesterona determinó que las ovejas probadas tuvieron un cuerpo luteo funcional al comienzo del experimento. Se concluye que el uso combinado de CIDR mas GnRH, 48 h antes y al momento del retiro del dispositivo, no tiene efecto sobre el comportamiento reproductivo en ovejas F1 (Damara x Merino).

          Translated abstract

          The experiment was carried out to evaluate the reproductive performance of F1 (Damara x Merino) ewes imported from Australia, synchronised with controlled internal drugs (CIDR) and two times of injection of gonadotropin releasing hormone (GnRH). Forty five F1 ewes at first lambing, of 18.08 ± .07 months of age and 43.3 ± 5.6 kg of body weight were randomly assigned to one of three treatments: T1 (n = 15): CIDR for 12 days; T2 (n = 15): CIDR for 12 days plus 25 μg of GnRH 48 h before CIDR withdrawal and T3 (n = 15): CIDR for 12 days plus 25 μg of GnRH at the moment of CIDR withdrawal. The percentage of estrus presentation (100%) was similar (p > 0.05) for T1, T2 and T3. The interval (h) of the onset of synchronised estrus after CIDR removal was different (p <0.05) for T2 and T3 (43.8 ± 5.4 and 45.7 ± 3.3 h) compared with T1 (37.3 ± 7.9 h). The percentage of fertility at the synchronised estrus was similar (p > 0.05) for T1, T2 and T3 (60.0, 53.3 y 46.6%, respectively). The general average prolificacy was 100.8% and similar (p > 0.05) among treatments. The concentration of progesterone insured that the tested ewes had a functional corpus luteum at the beginning of the experiment. It is concluded that the combined use of CIDR and GnRH, 48 h before and at the moment of CIDR removal, has no effect on the reproductive performance of F1 (Damara x Merino) ewes.

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            Evidence for GnRH Regulation by Leptin: Leptin Administration Prevents Reduced Pulsatile LH Secretion during Fasting

            Administration of leptin during undernutrition improves reproductive function, but whether this occurs at the level of the brain, pituitary, or gonads is not yet clear. The present study tested the hypothesis that one important mechanism is the control of pulsatile gonadotropin-releasing hormone (GnRH) secretion. Our approach was to determine if leptin could prevent the marked suppression of pulsatile luteinizing hormone (LH) secretion which occurs during fasting. Leptin (3 µg/g i.p.; three times/48 h) or vehicle was administered during a 48-hour fast in adult ovariectomized and estrogen-treated ovariectomized rats (n = 5–7/group). LH was measured in blood samples collected every 6 min for 2 h before and after fasting. In vehicle-treated animals, plasma insulin and leptin levels decreased after fasting. As expected, the LH pulse frequency also decreased markedly. When circulating leptin remained artificially elevated during fasting, the suppression of LH pulse frequency did not occur. Leptin treatment maintained a high LH pulse frequency in the presence or absence of estrogen. The finding that leptin modulates LH pulse frequency indicates that this fat-derived hormone conveys information about nutrition to mechanisms which regulate pulsatile gonadotropin-releasing hormone secretion. Because this occurs in the absence of estrogen, the mechanism does not necessarily involve modulation of negative feedback.
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              Synchronization of ovarian follicular waves with a gonadotropin-releasing hormone agonist to increase the precision of estrus in cattle: a review.

              Treatment with GnRH and PGF2 alpha is a practical method for controlling ovarian follicular and luteal functions and increasing the precision of estrus synchronization in cyclic and acyclic postpartum cows and heifers. This method reduces considerably the period of time needed for estrus detection; it synchronizes the estrous cycle of 70 to 80% of the cyclic cows to within a 4-d interval without any detrimental effect on the fertility rate (65 to 85%). Moreover, resumption of ovarian activity and normal fertility in acyclic cows in favored. Administration of GnRH eliminates the large follicles by ovulation or atresia and induces emergence of a new follicular wave within 3 to 4 d after treatment at any stage of the estrous cycle, but it limits further growth of these emerging follicles by increasing atresia. The precision of estrus and the unaltered fertility rate is due to the synchronized selection of a new larger growing follicle, which becomes the ovulatory follicle after PGF(2 alpha)-induced luteolysis 6 d after GnRH treatment. Also, fixed-time AI programs without the need for estrus detection may be possible using a second injection of GnRH in a GnRH-PGF(2 alpha)-GnRH protocol to ovulate the selected follicle at a precise time. We describe a physiological model to explain how the precision of estrus is improved following PGF(2 alpha)-induced luteolysis, via the effect of pretreatment with GnRH on follicular development and luteal functions in cattle. Application of this model to the development of reliable methods of fixed-time insemination is also explored.
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                Author and article information

                Journal
                uc
                Universidad y ciencia
                Universidad y ciencia
                Universidad Juárez Autónoma de Tabasco, Dirección de Investigación y Posgrado (Villahermosa, Tabasco, Mexico )
                0186-2979
                December 2008
                : 24
                : 3
                : 175-182
                Affiliations
                [03] Córdoba Veracruz orgnameColegio de Postgraduados orgdiv1Campus Córdoba
                [01] Tapachula Chiapas orgnamela Universidad Autónoma de Chiapas orgdiv1Facultad de Ciencias Agrícolas México jaimej@ 123456unach.mx
                [02] Texcoco estado de México orgnameColegio de Postgraduados, Montecillos orgdiv1Programa de Ganadería
                [04] orgnameResultados de la búsquedaUniversidad Autónoma del Estado de México orgdiv1Centro Universitario UAEM-Temascaltepec
                Article
                S0186-29792008000300001 S0186-2979(08)02400300001
                6a69c785-752b-4756-af4a-60006c9b1e0f

                This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 International License.

                History
                : 08 August 2008
                : 16 July 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 29, Pages: 8
                Product

                SciELO Mexico

                Categories
                Artículos

                progesterona,sincronización de estros,reproducción,ovejas,ewes,GnRH,CIDR,reproduction,estrus synchronisation,progesterone

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