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      A Simple and Rapid Method for Isolation of Retinal S Antigen

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          Abstract

          A procedure is described for the rapid isolation of S antigen from retinal tissue, by salt precipitation, gel filtration and final purification by adsorption chromatography on hydroxyapatite-agarose. The method yielded a highly purified material and allowed an excellent preservation of the antigenicity, as shown by high pathogenic activity in induction of experimental autoimmune uveoretinitis.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1982
          1982
          04 December 2009
          : 14
          : 4
          : 249-255
          Affiliations
          Laboratoire d’lmmunopathologie de l’Œil, Université P.- et-M.-Curie, Institut Biomédical des Cordeliers, Paris, France
          Article
          265199 Ophthalmic Res 1982;14:249–255
          10.1159/000265199
          6813787
          6a818891-8de6-4eb1-bee6-6b6e3d2db1d7
          © 1982 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 05 November 1981
          : 29 March 1982
          Page count
          Pages: 7
          Categories
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Hydroxyapatite-agarose,Autoimmunity,Retinal S antigen,Adsorption chromatography,Experimental autoimmune uveoretinitis,Autoantigen,Retina

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