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      Effect of Polyamines on Mesangial Cell Ecto-5′-Nucleotidase and Ecto-ATPase Activity

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          Polyamines were found to modulate the activity of several membrane-bound enzymes, participating in cell growth and differentiation. We have studied the effect of polyamines (spermidine, spermine and putrescine) on rat mesangial cell ectoenzymes: 5′-nucleotidase, Mg<sup>2+</sup>-ATPase and Ca<sup>2+</sup>-ATPase. Ecto-5′-nucleotidase activity was significantly increased after 48 h treatment with spermine and spermidine. Mg<sup>2+</sup>-ATPase was increased only after treatment with spermidine; however, Ca<sup>2+</sup>-ATPase was significantly increased after both spermine and spermidine treatment of mesangial cells. Culture of mesangial cells with putrescine did not change the activity of these ectoenzymes. Increased expression of mesangial cell ecto-ATPase and ecto-5′-nucleotidase after spermine and spermidine treatment could result in an increased production of adenosine, a powerful autacoid interesting with respect to a role of mesangial cells in inflammatory processes.

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          Formation of a Complex Containing ATP, Mg2+, and Spermine


            Author and article information

            S. Karger AG
            September 2002
            14 August 2002
            : 92
            : 1
            : 216-218
            Institute of Nephrology and Hemodialysis, Faculty of Medicine, Niš, Yugoslavia
            64466 Nephron 2002;92:216–218
            © 2002 S. Karger AG, Basel

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            Page count
            Figures: 1, References: 8, Pages: 3
            Self URI (application/pdf): https://www.karger.com/Article/Pdf/64466
            Short Communication

            Cardiovascular Medicine, Nephrology

            Ecto-5′-nucleotidase, Ecto-ATPase, Polyamines, Mesangial cells


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