Morphometric evaluation of bone regeneration in segmental mandibular bone defects filled with bovine bone xenografts in a split-mouth rabbit model

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Bovine bone grafts have been widely used in dentistry for guided tissue regeneration and can support new bone formation in direct contact with the graft. The aim of this study was to compare the morphometric and bone density changes after using two different bovine bone graft blocks in segmental osseous defects in the mandible of rabbits following different postoperative periods.

Material and methods

Critical size segmental defects were surgically created bilaterally in the jaw of 18 rabbits. The defects were filled with either deproteinized bovine bone mineral with 10% collagen (DBBM-C; BioOss Collagen®), lyophilized bovine medullary bone (LBMB; Orthogen®), or left untreated according to a split-mouth design. Animals were sacrificed after 3 or 6 months of healing. The hemimandibles were scanned ex vivo using a high-resolution (19 μm) microcomputed tomography. Morphometric and bone density parameters were calculated in the region of the defect using CT-Analyser (Bruker). Initial graft blocks were used as baseline.

Results

DBBM-C presented a denser microarchitecture, in comparison to LBMB at baseline. DBBM-C and LBMB grafted regions showed a similar progressive remodeling, with a significant decrease in structure complexity and maintenance of bone volume fraction during the postoperative follow-up periods. Both graft materials showed an enhanced bone replacement and more complex structure compared to untreated defects. The apparent fusion between the graft and host bone was observed only in the defects filled with LBMB.

Conclusion

LBMB grafts showed a similar behavior as DBBM-C regarding structural remodeling. In LBMB samples, apparent integration between the host bone and the graft was present.

Related collections

Most cited references 27

Record: found
Abstract: found
Article: not found

Three-dimensional plotted scaffolds with controlled pore size gradients: Effect of scaffold geometry on mechanical performance and cell seeding efficiency.

Silmara Sousa, Lays Sobral, João F. Mano … (2011)

Scaffolds produced by rapid prototyping (RP) techniques have proved their value for tissue engineering applications, due to their ability to produce predetermined forms and structures featuring fully interconnected pore architectures. Nevertheless, low cell seeding efficiency and non-uniform distribution of cells remain major limitations when using such types of scaffold. This can be mainly attributed to the inadequate pore architecture of scaffolds produced by RP and the limited efficiency of cell seeding techniques normally adopted. In this study we aimed at producing scaffolds with pore size gradients to enhance cell seeding efficiency and control the spatial organization of cells within the scaffold. Scaffolds based on blends of starch with poly(ε-caprolactone) featuring both homogeneously spaced pores (based on pore sizes of 0.75 and 0.1 mm) and pore size gradients (based on pore sizes of 0.1-0.75-0.1 and 0.75-0.1-0.75 mm) were designed and produced by three-dimensional plotting. The mechanical performance of the scaffolds was characterized using dynamic mechanical analysis (DMA) and conventional compression testing under wet conditions and subsequently characterized using scanning electron microscopy and micro-computed tomography. Osteoblast-like cells were seeded onto such scaffolds to investigate cell seeding efficiency and the ability to control the zonal distribution of cells upon seeding. Scaffolds featuring continuous pore size gradients were originally produced. These scaffolds were shown to have intermediate mechanical and morphological properties compared with homogenous pore size scaffolds. The pore size gradient scaffolds improved seeding efficiency from ∼35% in homogeneous scaffolds to ∼70% under static culture conditions. Fluorescence images of cross-sections of the scaffolds revealed that scaffolds with pore size gradients induce a more homogeneous distribution of cells within the scaffold. Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

0 comments Cited 131 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Current bone substitutes for implant dentistry.

Masahiro Yamada, Hiroshi Egusa (2018)

Alveolar ridge augmentation is essential for success in implant therapy and depends on the biological performance of bone graft materials. This literature review aims to comprehensively explain the clinically relevant capabilities and limitations of currently available bone substitutes for bone augmentation in light of biomaterial science.

0 comments Cited 62 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

In vivo bone response to 3D periodic hydroxyapatite scaffolds assembled by direct ink writing.

Maya A. Yampolsky, James Smay, Whitney Simon … (2007)

The in vivo bone response of 3D periodic hydroxyapatite (HA) scaffolds is investigated. Two groups of HA scaffolds (11 mm diameter x 3.5 mm thick) are fabricated by direct-write assembly of a concentrated HA ink. The scaffolds consist of cylindrical rods periodically arranged into four quadrants with varying separation distances between rods. In the first group, HA rods (250 microm in diameter) are patterned to create pore channels, whose areal dimensions are 250 x 250 microm(2) in quadrant 1, 250 x 500 microm(2) in quadrants 2 and 4, and 500 x 500 microm(2) in quadrant 3. In the second group, HA rods (400 microm in diameter) are patterned to create pore channels, whose areal dimensions of 500 x 500 microm(2) in quadrant 1, 500 x 750 microm(2) in quadrants 2 and 4, and 750 x 750 microm(2) in quadrant 3. Each group of scaffolds is partially densified by sintering at 1200 degrees C prior to being implanted bilaterally in trephine defects of skeletally mature New Zealand White rabbits. Their tissue response is evaluated at 8 and 16 weeks using micro-computed tomography, histology, and scanning electron microscopy. New trabecular bone is conducted rapidly and efficiently across substantial distances within these patterned 3D HA scaffolds. Our observations suggest that HA rods are first coated with a layer of new bone followed by subsequent scaffold infilling via outward and inward radial growth of the coated regions. Direct-write assembly of 3D periodic scaffolds composed of micro-porous HA rods arrayed to produce macro-pores that are size-matched to trabecular bone may represent an optimal strategy for bone repair and replacement structures. Copyright 2007 Wiley Periodicals, Inc.

0 comments Cited 40 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Mariana Quirino Silveira Soares: +55 14 32358254 , marianaqsilveira@usp.br

Journal

Journal ID (nlm-ta): Int J Implant Dent

Journal ID (iso-abbrev): Int J Implant Dent

Title: International Journal of Implant Dentistry

Publisher: Springer Berlin Heidelberg (Berlin/Heidelberg )

ISSN (Electronic): 2198-4034

Publication date (Electronic): 10 September 2019

Publication date PMC-release: 10 September 2019

Publication date Collection: December 2019

Volume: 5

Electronic Location Identifier: 32

Affiliations

[1 ]ISNI 0000 0004 1937 0722, GRID grid.11899.38, Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, , University of São Paulo, ; Bauru, 17012-901 Brazil

[2 ]ISNI 0000 0004 0626 3338, GRID grid.410569.f, OMFS-IMPATH research group, Department of Imaging and Pathology, Faculty of Medicine, , KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, ; Leuven, Belgium

[3 ]ISNI 0000 0004 1937 0626, GRID grid.4714.6, Department of Dental Medicine, , Karolinska Institutet, ; Stockholm, Sweden

[4 ]ISNI 0000 0004 1937 0722, GRID grid.11899.38, Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, , University of São Paulo, ; Bauru, Brazil

Article

Publisher ID: 187

DOI: 10.1186/s40729-019-0187-1

PMC ID: 6733940

PubMed ID: 31502091

SO-VID: 6aa0c1e8-a913-4fc9-9428-9472cb234776

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

History

Date received : 28 February 2019

Date accepted : 23 August 2019

Funding

Funded by: CAPES

Award ID: 001

Award Recipient : Mariana Quirino Silveira Soares

Funded by: Research Foundation Flanders

Award ID: ZKC8367

Custom metadata

Keywords: microcomputed tomography,alveolar bone grafting,bone morphometry,deproteinized bovine bone mineral

Data availability:

Keywords: microcomputed tomography, alveolar bone grafting, bone morphometry, deproteinized bovine bone mineral

Morphometric evaluation of bone regeneration in segmental mandibular bone defects filled with bovine bone xenografts in a split-mouth rabbit model

Read this article at

Abstract

Background

Material and methods

Results

Conclusion

Related collections

Animal models of intrauterine growth restriction (IUGR)

Most cited references 27

Three-dimensional plotted scaffolds with controlled pore size gradients: Effect of scaffold geometry on mechanical performance and cell seeding efficiency.

Current bone substitutes for implant dentistry.

In vivo bone response to 3D periodic hydroxyapatite scaffolds assembled by direct ink writing.

Author and article information

Contributors

Journal

Affiliations

Article

History

Funding

Categories

Custom metadata

Comments

Comment on this article

Similar content 79

Cited by 3

Most referenced authors 381