Jennifer C Miller 1 , 2 , Brian D. Brown 1 , 3 , Tal Shay 4 , Emmanuel L Gautier 1 , 5 , 6 , Vladimir Jojic 7 , Ariella Cohain 3 , Gaurav Pandey 3 , Marylene Leboeuf 1 , 2 , Kutlu G Elpek 9 , Julie Helft 1 , 2 , Daigo Hashimoto 1 , 2 , Andrew Chow 1 , 2 , 10 , Jeremy Price 1 , 2 , Melanie Greter 1 , 2 , 7 , Milena Bogunovic 1 , 2 , Angelique Bellemare-Pelletier 9 , Paul S. Frenette 10 , Gwendalyn J. Randolph 1 , 5 , 6 , Shannon J. Turley 9 , Miriam Merad 1 , 2 , the Immunological Genome Consortium
15 July 2012
Although, much progress has been made in our understanding of DC ontogeny and function, the transcriptional regulation of DC lineage commitment and functional specialization in vivo is poorly understood. We performed a comprehensive comparative analysis of CD8+, CD103+, CD11b+, and plasmacytoid DC subsets and the recently identified Macrophage DC precursors and Common DC precursors across the entire immune system. Here we characterize candidate transcriptional activators involved in myeloid progenitor commitment to the DC lineage and predicted regulators of DC functional diversity in tissues. We identify a molecular signature that distinguishes tissue DC from macrophages. We also identify a transcriptional program expressed specifically during steady-state tissue DC migration to the draining lymph nodes that may control tolerance to self-tissue antigens.