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      EVALUACIÓN DE BACTERIOCINAS COMO MEDIO PROTECTOR PARA LA BIOPRESERVACIÓN DE LA CARNE BAJO REFRIGERACIÓN Translated title: EVALUATION OF BACTERIOCINES AS PROTECTIVE MEANS FOR THE BIOPRESERVATION OF REFRIGERATED MEAT

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          Abstract

          El objetivo del presente trabajo fue evaluar la extensión de la vida útil de carne refrigerada mediante la aplicación de extracto crudo de bacteriocinas producido por Lactobacillus plantarum LPBM10. Se utilizaron filetes de solomo redondo (longissimus dorsi) almacenados durante 12 días a 3º C y analizados por medio de análisis microbiológicos, pH, textura, pérdidas de peso y sensoriales. Se encontraron diferencias significativas entre los tratamientos para psicrotrófilos y coliformes totales, resultando mejor el tratamiento con extracto crudo de bacteriocinas que tuvo efecto bactericida. Los coliformes fecales también fueron inhibidos por el extracto crudo de bacteriocinas. Los cambios de pH y pérdida de peso presentan variaciones estadísticas entre tratamientos y entre días. La fuerza de corte disminuyó durante el tiempo de almacenamiento en todos los tratamientos mejorando la terneza de la carne sin presentar diferencia significativa. La apariencia, color y aroma disminuyeron el valor de aceptación a medida que avanzo el tiempo.

          Translated abstract

          The objective was to evaluate shelf-life extension of fresh beef meat applying crude extract of bacteriocines produced by a native strain of Lactobacillus plantarum LPBM10. Fillets of rib (longissimus dorsi) were stocked during 12 days at 3° C and analyzed by means of microbiology analysis, pH, texture, weight loss and sensorial. Significant differences were found among treatments for psychrotrophics and fecal coliforms, being better the treatment with crude bacteriocin extract that had germicide effect. Fecal coliforms were inhibited by the crude bacteriocin extract during most of the period. The mesophilic count didnit present difference among treatments. The pH changes and the weight loss present statistical variations among treatments and between days. Cutting force diminished during storage time in all treatments improving meat tenderness without presenting significant difference. The appearance, color and aroma diminished the acceptance value as the storage time advanced.

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          Bacteriocin-based strategies for food biopreservation.

          Bacteriocins are ribosomally-synthesized peptides or proteins with antimicrobial activity, produced by different groups of bacteria. Many lactic acid bacteria (LAB) produce bacteriocins with rather broad spectra of inhibition. Several LAB bacteriocins offer potential applications in food preservation, and the use of bacteriocins in the food industry can help to reduce the addition of chemical preservatives as well as the intensity of heat treatments, resulting in foods which are more naturally preserved and richer in organoleptic and nutritional properties. This can be an alternative to satisfy the increasing consumers demands for safe, fresh-tasting, ready-to-eat, minimally-processed foods and also to develop "novel" food products (e.g. less acidic, or with a lower salt content). In addition to the available commercial preparations of nisin and pediocin PA-1/AcH, other bacteriocins (like for example lacticin 3147, enterocin AS-48 or variacin) also offer promising perspectives. Broad-spectrum bacteriocins present potential wider uses, while narrow-spectrum bacteriocins can be used more specifically to selectively inhibit certain high-risk bacteria in foods like Listeria monocytogenes without affecting harmless microbiota. Bacteriocins can be added to foods in the form of concentrated preparations as food preservatives, shelf-life extenders, additives or ingredients, or they can be produced in situ by bacteriocinogenic starters, adjunct or protective cultures. Immobilized bacteriocins can also find application for development of bioactive food packaging. In recent years, application of bacteriocins as part of hurdle technology has gained great attention. Several bacteriocins show additive or synergistic effects when used in combination with other antimicrobial agents, including chemical preservatives, natural phenolic compounds, as well as other antimicrobial proteins. This, as well as the combined use of different bacteriocins may also be an attractive approach to avoid development of resistant strains. The combination of bacteriocins and physical treatments like high pressure processing or pulsed electric fields also offer good opportunities for more effective preservation of foods, providing an additional barrier to more refractile forms like bacterial endospores as well. The effectiveness of bacteriocins is often dictated by environmental factors like pH, temperature, food composition and structure, as well as the food microbiota. Foods must be considered as complex ecosystems in which microbial interactions may have a great influence on the microbial balance and proliferation of beneficial or harmful bacteria. Recent developments in molecular microbial ecology can help to better understand the global effects of bacteriocins in food ecosystems, and the study of bacterial genomes may reveal new sources of bacteriocins.
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            A comparison between two methods (Warner-Bratzler and texture profile analysis) for testing either raw meat or cooked meat.

            Two methods for assessing texture characteristics of meat (Warner-Bratzler (WB) - and texture profile analysis (TPA)), both performed either on raw or on cooked meat, were tested in 96 samples of m. longissimus dorsi muscle of eight heifers and eight bulls, aged 1, 3 or 6 days post-mortem. A sensory analysis was also performed on 96 samples. Sensory variates were predicted by instrumental variates as follows: hardness was better predicted by TPA than by WB; springiness was only predicted by WB; juiciness was only significantly predicted by TPA; greasiness was always poorly predicted, but the prediction was better with TPA, and the number of chewings was also better predicted with TPA. Results suggested the convenience of performing a TPA for assessing meat texture as, in cooked meat, only TPA furnished highly significant correlations for hardness, for juiciness and for the number of chewings. Although WB could predict hardness and springiness, only the equation for the prediction of the number of chewings was useful (r(2)=0.171, P<0.004). It seems that texture parameters, assessed by a TPA and performed on cooked meat, are the best predictors of sensory texture in bovine meat.
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              Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram-negative bacteria

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                Author and article information

                Journal
                rchnut
                Revista chilena de nutrición
                Rev. chil. nutr.
                Sociedad Chilena de Nutrición, Bromatología y Toxicología (Santiago, , Chile )
                0717-7518
                September 2009
                : 36
                : 3
                : 228-238
                Affiliations
                [02] Medellín orgnameUniversidad Nacional de Colombia orgdiv1Facultad de Ciencias Colombia
                [01] Medellín orgnameUniversidad Nacional de Colombia orgdiv1Facultad de Ciencias Agropecuarias orgdiv2Departamento de Ingeniería Agrícola y de Alimentos Colombia
                Article
                S0717-75182009000300005 S0717-7518(09)03600300005
                10.4067/S0717-75182009000300005
                6b1e116e-dea3-4de3-85aa-6f308fa286c1

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 20 June 2009
                : 10 March 2009
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 11
                Product

                SciELO Chile

                Categories
                ARTÍCULOS ORIGINALES

                LPBM10,biopreservation,lactobacillus plantarum,carne,biopreservación,refrigeración,bacteriocins,bacteriocinas,plantarum

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