+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: not found

      Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe 3+ and bioimaging both in vitro and in vivo

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe 3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.


          Intracellular Fe 3+ amount is one of the critical determinants of human health. The development of simple and effective probes for the quantitative detection of Fe 3+ in vivo is of great significance for the early diagnosis of disease or disorder associated with iron deficiency or overload. In this study, remarkable carbon dots, which can serve as a biosensor for efficient intracellular Fe 3+ detection, were synthesized by hydrothermal carbonization of Fusobacterium nucleatum, an anaerobic bacterium. The achieved F. nucleatum-carbon dots (Fn-CDs) possessed the features of strong fluorescence, high stability and excellent biocompatibility. The obtained Fn-CDs could easily internalize into both plant cells and human cells with excellent ability for cell tracking and biomedical labeling. The fluorescence of Fn-CDs could still remain for another 24 hours after penetrating into cells. Furthermore, the fluorescent Fn-CDs were very sensitive to the presence of Fe 3+ ions even in cells, exhibiting great promising applications in in vivo detection of Fe 3+ ions. In addition, the Fn-CDs posed no harm to the mice, being circulated and excreted within a short time, making the Fn-CDs an excellent candidate for bioimaging and biosensing in vivo.

          Related collections

          Most cited references 37

          • Record: found
          • Abstract: found
          • Article: not found

          Preparation of highly photoluminescent sulfur-doped carbon dots for Fe(iii) detection

          Sulfur-doped carbon dots were synthesized by a one-step hydrothermal method and exhibited high fluorescence quantum yield (67%) and exceptional emission behavior. Sulfur-doped carbon dots (S-doped C-dots)were synthesized using a simple and straightforward hydrothermal method. The as-prepared S-doped C-dots exhibit significant fluorescence quantum yield (67%) and unique emission behavior. The spherical S-doped C-dots have an average diameter of 4.6 nm and the fluorescence of S-doped C-dots can be effectively and selectively quenched by Fe 3+ ions. Thus, S-doped C-dots were applied as probes toward Fe 3+ detection, exhibiting a limit of detection of 0.1 μM.
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Out of Balance—Systemic Iron Homeostasis in Iron-Related Disorders

            Iron is an essential element in our daily diet. Most iron is required for the de novo synthesis of red blood cells, where it plays a critical role in oxygen binding to hemoglobin. Thus, iron deficiency causes anemia, a major public health burden worldwide. On the other extreme, iron accumulation in critical organs such as liver, heart, and pancreas causes organ dysfunction due to the generation of oxidative stress. Therefore, systemic iron levels must be tightly balanced. Here we focus on the regulatory role of the hepcidin/ferroportin circuitry as the major regulator of systemic iron homeostasis. We discuss how regulatory cues (e.g., iron, inflammation, or hypoxia) affect the hepcidin response and how impairment of the hepcidin/ferroportin regulatory system causes disorders of iron metabolism.
              • Record: found
              • Abstract: not found
              • Article: not found

              One-step hydrothermal approach to fabricate carbon dots from apple juice for imaging of mycobacterium and fungal cells


                Author and article information

                Analytical Methods
                Anal. Methods
                Royal Society of Chemistry (RSC)
                March 11 2021
                : 13
                : 9
                : 1121-1131
                [1 ]Faculty of Life Science and Technology
                [2 ]Kunming University of Science and Technology
                [3 ]Kunming 650500
                [4 ]China
                [5 ]Department of Education
                [6 ]Yunnan Minzu University
                [7 ]State Key Laboratory of Phytochemistry and Plant Resources in West China
                [8 ]Kunming Institute of Botany
                [9 ]Chinese Academy of Sciences
                [10 ]Kunming
                [11 ]Department of Hematology
                [12 ]The Second People's Hospital of Yunnan Province
                [13 ]The Affiliated Hospital of Yunnan University
                [14 ]Shenzhen Institute of Respiratory Diseases
                [15 ]The Second Clinical Medical College
                [16 ]Jinan University (Shenzhen People's Hospital)
                [17 ]Shenzhen 518020
                © 2021
                Self URI (article page): http://xlink.rsc.org/?DOI=D1AY00020A


                Comment on this article