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      A Novel Approach for Forensic Differentiation of Grass Stains Using ATR FT-IR Spectroscopy and Chemometrics

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      Arab Journal of Forensic Sciences & Forensic Medicine
      Naif Arab University for Security Sciences

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          Abstract

          Grass evidence often encountered at the crime scene, helps in establishing the primary as well as secondary crime scenes. Due to the limited quantity and intricate nature of samples, there is a need for fast, sensitive and reliable techniques for the effective analysis of this vital evidence. In the current study, an attempt is made to study the feasibility of ATR FT-IR in combination with chemometric tools for chemical characterization and species differentiation of grass stains. Ten different grass species belonging to three subfamilies, Panicoideae, Chlorodoidae, and Bambusoideae, were collected and analyzed by using the ATR FT-IR spectroscopy combined with the PLSR chemometric tool. Results showed a clear difference between samples of Panicoideae, Chlorodoidae, and Bambusoideae subfamilies, and between species of each subfamily. To analyse the performance of the classification model, a set of 10 unknown/blind samples (unknown to the analyst) were randomly selected from the training dataset and all unknown/blind samples were accurately assigned in their corresponding group. ATR FT-IR spectroscopy requires minimal sample preparation and can be successfully used as an eco-friendly, non-destructive, and reliable alternative to other existing methods.

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          Author and article information

          Journal
          Arab Journal of Forensic Sciences & Forensic Medicine
          AJFSFM
          Naif Arab University for Security Sciences
          16586794
          16586786
          December 15 2020
          November 05 2020
          : 2
          : 2
          : 128-139
          Article
          10.26735/QMFZ6158
          6b6ac928-8b2b-4075-b90a-e4482890fe8e
          © 2020

          This work is licensed under Attribution-NonCommercial 4.0 International license. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/


          Entomology,Pathology,Forensic science,Criminology,Dentistry,Anthropology
          Entomology, Pathology, Forensic science, Criminology, Dentistry, Anthropology

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