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      Hypertension in Unilaterally Nephrectomized Rats Induced by Single–Kidney Transfection with Angiotensinogen cDNA

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          Plasmid expression vectors containing angiotensinogen (ATG) cDNA were complexed to cationic liposomes and injected into the renal artery of unilaterally nephrectomized rats to evaluate the effect of intrarenal ATG cDNA on arterial blood pressure and the renin–angiotensin system. Systolic blood pressures measured by tail cuff on days 12, 16, and 18 after transfection were significantly higher in rats that received ATG cDNA than in control rats that received the lac Z reporter gene. Plasma renin activity and plasma ATG concentration were unchanged. These results provide direct evidence that the availability of intrarenal ATG may be instrumental in the development of systemic hypertension.

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          Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

          A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

            Author and article information

            Kidney Blood Press Res
            Kidney and Blood Pressure Research
            S. Karger AG
            15 October 1999
            : 23
            : 1
            : 42-48
            Departments of aPhysiology and bExercise and Sports Science, Colorado State University, Fort Collins, Colo., USA
            25953 Kidney Blood Press Res 2000;23:42–48
            © 1999 S. Karger AG, Basel

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            Figures: 4, Tables: 2, References: 36, Pages: 7
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