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      Poisson-Nernst-Planck equations in a ball

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          Abstract

          The Poisson Nernst-Planck equations for charge concentration and electric potential in a ball is a model of electro-diffusion of ions in the head of a neuronal dendritic spine. We study the relaxation and the steady state when an initial charge of ions is injected into the ball. The steady state equation is similar to the Liouville-Gelfand-Bratu-type equation with the difference that the boundary condition is Neumann, not Dirichlet and there a minus sign in the exponent of the exponential term. The entire boundary is impermeable to the ions and the electric field satisfies the compatibility condition of Poisson's equation. We construct a steady radial solution and find that the potential is maximal in the center and decreases toward the boundary. We study the limit of large charge in dimension 1,2 and 3. For the case of a small absorbing window in the sphere, we find the escape rate of an ion from the steady density.

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          Most cited references19

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          Dendritic spines as basic functional units of neuronal integration.

          Most excitatory synaptic connections occur on dendritic spines. Calcium imaging experiments have suggested that spines constitute individual calcium compartments, but recent results have challenged this idea. Using two-photon microscopy to image fluorescence with high resolution in strongly scattering tissue, we measured calcium dynamics in spines from CA1 pyramidal neurons in slices of rat hippocampus. Subthreshold synaptic stimulation and spontaneous synaptic events produced calcium accumulations that were localized to isolated spines, showed stochastic failure, and were abolished by postsynaptic blockers. Single somatic spikes induced fast-peaking calcium accumulation in spines throughout the cell. Pairing of spikes with synaptic stimulation was frequently cooperative, that is, it resulted in supralinear calcium accumulations. We conclude: (1) calcium channels exist in spine heads; (2) action potentials invade the spines; (3) spines are individual calcium compartments; and (4) spines can individually detect the temporal coincidence of pre- and postsynaptic activity, and thus serve as basic functional units of neuronal integration.
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            The narrow escape problem for diffusion in cellular microdomains.

            The study of the diffusive motion of ions or molecules in confined biological microdomains requires the derivation of the explicit dependence of quantities, such as the decay rate of the population or the forward chemical reaction rate constant on the geometry of the domain. Here, we obtain this explicit dependence for a model of a Brownian particle (ion, molecule, or protein) confined to a bounded domain (a compartment or a cell) by a reflecting boundary, except for a small window through which it can escape. We call the calculation of the mean escape time the narrow escape problem. This time diverges as the window shrinks, thus rendering the calculation a singular perturbation problem. Here, we present asymptotic formulas for the mean escape time in several cases, including regular domains in two and three dimensions and in some singular domains in two dimensions. The mean escape time comes up in many applications, because it represents the mean time it takes for a molecule to hit a target binding site. We present several applications in cellular biology: calcium decay in dendritic spines, a Markov model of multicomponent chemical reactions in microdomains, dynamics of receptor diffusion on the surface of neurons, and vesicle trafficking inside a cell.
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              Direct measurement of coupling between dendritic spines and shafts.

              Characterization of the diffusional and electrotonic coupling of spines to the dendritic shaft is crucial to understanding neuronal integration and synaptic plasticity. Two-photon photobleaching and photorelease of fluorescein dextran were used to generate concentration gradients between spines and shafts in rat CA1 pyramidal neurons. Diffusional reequilibration was monitored with two-photon fluorescence imaging. The time course of reequilibration was exponential, with time constants in the range of 20 to 100 milliseconds, demonstrating chemical compartmentalization on such time scales. These values imply that electrical spine neck resistances are unlikely to exceed 150 megohms and more likely range from 4 to 50 megohms.
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                Author and article information

                Journal
                2015-05-07
                Article
                1505.02173
                6bda921f-6313-4f37-86fb-d36b9e70d6af

                http://arxiv.org/licenses/nonexclusive-distrib/1.0/

                History
                Custom metadata
                35Q82, 35Q84, 82C31, 35J91
                20 pages in SIAP 2015
                physics.class-ph cond-mat.stat-mech math.AP q-bio.SC

                Analysis,Condensed matter,Cell biology,Classical mechanics
                Analysis, Condensed matter, Cell biology, Classical mechanics

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