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      The NB-LRR gene Pm60 confers powdery mildew resistance in wheat

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          Abstract

          Powdery mildew is one of the most devastating diseases of wheat. To date, few powdery mildew resistance genes have been cloned from wheat due to the size and complexity of the wheat genome. Triticum urartu is the progenitor of the A genome of wheat and is an important source for powdery mildew resistance genes. Using molecular markers designed from scaffolds of the sequenced T. urartu accession and standard map-based cloning, a powdery mildew resistance locus was mapped to a 356-kb region, which contains two nucleotide-binding and leucine-rich repeat domain (NB-LRR) protein-encoding genes. Virus-induced gene silencing, single-cell transient expression, and stable transformation assays demonstrated that one of these two genes, designated Pm60, confers resistance to powdery mildew. Overexpression of full-length Pm60 and two allelic variants in Nicotiana benthamiana leaves induced hypersensitive cell death response, but expression of the coiled-coil domain alone was insufficient to induce hypersensitive response. Yeast two-hybrid, bimolecular fluorescence complementation and luciferase complementation imaging assays showed that Pm60 protein interacts with its neighboring NB-containing protein, suggesting that they might be functionally related. The identification and cloning of this novel wheat powdery mildew resistance gene will facilitate breeding for disease resistance in wheat.

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          Author and article information

          Journal
          New Phytologist
          New Phytol
          Wiley
          0028646X
          April 2018
          April 2018
          December 27 2017
          : 218
          : 1
          : 298-309
          Affiliations
          [1 ]State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests; Fujian Agriculture and Forestry University; Fuzhou 350002 China
          [2 ]Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops; Plant Immunity Center; Fujian Agriculture and Forestry University; Fuzhou 350002 China
          [3 ]State Key Laboratory of Plant Cell and Chromosome Engineering; Institute of Genetics and Development Biology; Chinese Academy of Sciences; Beijing 100101 China
          [4 ]State Key Laboratory of Plant Genomics; Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101 China
          [5 ]University of Chinese Academy of Sciences; Beijing 100049 China
          Article
          10.1111/nph.14964
          29281751
          6bdbf686-1554-48fe-87fe-b5352a0d155a
          © 2017

          http://doi.wiley.com/10.1002/tdm_license_1.1

          http://onlinelibrary.wiley.com/termsAndConditions#vor

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