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      Oxidative Denaturation of Lens Protein: Prevention by Pyruvate

      Ophthalmic Research
      S. Karger AG
      Protein carbonyl, Pyruvate, Oxygen radicals, Peroxide, Lens

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          The denaturation of lens proteins as apparent by the generation of protein carbonyl in the presence of active oxygen and the prevention of such denaturation by pyruvate were studied. Active oxygen was generated by the action of xanthine oxidase on xanthine under aerobic conditions. Rat lens protein when incubated with xanthine and xanthine oxidase produced significant amounts of the carbonyl derivative. The formation of such carbonyl was substantially inhibited by pyruvate. In addition, the keto acid also was found to stimulate the utilization of glucose through HMP shunt, a mechanism known to transport reducing equivalents from glucose to peroxide. The results suggest that pyruvate exerts a beneficial effect in attenuating the age-related protein modifications and consequent physiological impairments. These studies are also considered useful from the therapeutic point of view.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 27
          : 1
          : 18-22
          Department of Ophthalmology, University of Maryland at Baltimore, Md., USA
          267562 Ophthalmic Res 1995;27:18–22
          © 1995 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 30 August 1994
          : 28 October 1994
          Page count
          Pages: 5
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Pyruvate,Protein carbonyl,Oxygen radicals,Peroxide,Lens


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