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      Novel Spectrophotometric Method for the Assay of Captopril in Dosage Forms using 2,6-Dichloroquinone-4-Chlorimide

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          Abstract

          A simple spectrophotometric method was developed for the determination of captopril (CPL) in pharmaceutical preparations. The method is based on coupling captopril with 2,6-dichloroquinone-4-chlorimide (DCQ) in dimethylsulphoxide. The yellow reaction product was measured at 443 nm. The absorbance–concentration plot was rectilinear over the range of 10-50 μg/mL with minimum detection limit (LOD) of 0.66 μg/mL and a quantification limit (LOQ) of 2.0 μg/mL. The different experimental parameters affecting the development and stability of the color were carefully studied and optimized. The proposed method was successfully applied to the analysis of commercial tablets and the results were in good agreement with those obtained using official and reference spectrophotometric methods. Hydrochlorothiazide which is frequently co-formulated with CPL did not interfere with the assay. A proposal of the reaction pathway was presented.

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          Direct spectrophotometric measurement of angiotensin I-converting enzyme inhibitory activity for screening bioactive peptides.

          Huan Liu, Hui Shi, Guo-Wei Le … (2005)
          A direct, extraction-free spectrophotometric assay was developed for determination of angiotensin I-converting enzyme activity (ACE) in the presence of ACE inhibitors using hippuryl-l-histidyl-l-leucine (HHL) as the ACE-specific substrate. This method relies on previously published spectrophotometric determination of hippuric acid (HA) content in the urine, the method of which was based on the specific colorimetric reaction of HA with benzene sulfonyl chloride (BSC) in the presence of quinoline. The proposed ACE inhibition assay was applied to the measurement of the ACE inhibitory activity of Captopril. IC(50) value of Captopril corresponded well with literature data. Furthermore, Alcalase hydrolysates of mung bean and rice protein isolates were assessed for ACE inhibitory activity by this method. These two hydrolysates showed high ACE inhibitory activity. This method proposed here was shown to be direct, sensitive, accurate, reproducible, and less expensive without separation of HA from ACE reaction mixture, and can be used for the screening of ACE inhibitory peptides derived from food proteins.
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            Advanced physico-chemical experiments

            J Rose (1964)
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              Simultaneous determination of captopril and hydrochlorothiazide in human plasma by reverse-phase HPLC from linear gradient elution.

              G Duan, Guo-zhong He, L. Shao … (2006)
              A simple, rapid and sensitive high-performance liquid chromatographic method for the simultaneous determination of captopril and hydrochlorothiazide in human plasma samples was developed. Captopril was derivatized with 2,4'-dibromoacetophenone (pBPB) to form a captopril-pBPB adduct. From acidified serum plasma samples, the hydrochlorothiazide and derivatized captopril was extracted with 5 ml ether, then with 5 ml dichloromethane. Effective chromatographic separation was achieved using a C(18) column (DIAMONSIL 150 mmx4 mm i.d., 5 microm) based on an acetonitrile-trifluoroacetic acid-water gradient elution at a flow rate of 1.2 ml/min. The internal standard (IS), derivatized captopril and hydrochlorothiazide were detected at 263 nm and were eluted at 4.2, 6.8 and 16.9 min, respectively. No endogenous substances were found to interfere. The limit of quantification for hydrochlorothiazide and derivatized captopril in plasma were 3.3 and 7 ng/ml. The calibration curve for derivatized captopril showed linearity in the range 20-4000 ng/ml, with a regression coefficient corresponding to 0.9993 and the coefficient of the variation of the points of the calibration curve being lower than 10%. The calibration curve for hydrochlorothiazide showed linearity in the range 10-1200 ng/ml, with a regression coefficient corresponding to 0.9999 and the coefficient of the variation of the points of the calibration curve being lower than 10%. The method was suitably validated and successfully applied to the determination of captopril and hydrochlorothiazide in human plasma samples.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Int J Biomed Sci
                Journal ID (iso-abbrev): Int J Biomed Sci
                Journal ID (publisher-id): IJBS
                Title: International Journal of Biomedical Science : IJBS
                Publisher: Master Publishing Group
                ISSN (Print): 1550-9702
                ISSN (Electronic): 1555-2810
                Publication date (Print): June 2008
                Volume: 4
                Issue: 2
                Pages: 147-154
                Affiliations
                [1 ] Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt;
                [2 ] Department of Analytical Chemistry, Faculty of Pharmacy, University of Helwan, Cairo, Egypt
                Author notes
                Corresponding author: Nahed El-Enany, Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt. Fax: +20502247496; E-mail: nelenany1@ 123456yahoo.com .
                Article
                Publisher ID: IJBS-4-147
                PMC ID: 3614690
                PubMed ID: 23675082
                SO-VID: 6d285657-fb8d-482a-9c3e-543201dfaac5
                Copyright © © Nahed El-Enany et al. Licensee Master Publishing Group
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 24 February 2008
                Date accepted : 19 March 2008
                Categories
                Subject: Article

                Keywords: 2,6-dichloroquinone-4-chlorimide (dcq),dosage forms,spectrophotometry,captopril
                Data availability:
                Keywords: 2,6-dichloroquinone-4-chlorimide (dcq), dosage forms, spectrophotometry, captopril

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