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      Evaluation of Flinders Technology Associates cards for storage and molecular detection of avian metapneumoviruses.

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          Abstract

          The feasibility of using Flinders Technology Associates (FTA) cards for the molecular detection of avian metapneumovirus (aMPV) by reverse transcriptase-polymerase chain reaction (RT-PCR) was investigated. Findings showed that no virus isolation was possible from aMPV-inoculated FTA cards, confirming viral inactivation upon contact with the cards. The detection limits of aMPV from the FTA card and tracheal organ culture medium were 10(1.5) median ciliostatic doses/ml and 10(0.75) median ciliostatic doses/ml respectively. It was possible to perform molecular characterization of both subtypes A and B aMPV using inoculated FTA cards stored for up to 60 days at 4 to 6°C. Tissues of the turbinate, trachea and lung of aMPV-infected chicks sampled either by direct impression smears or by inoculation of the tissue homogenate supernatants onto the FTA cards were positive by RT-PCR. However, the latter yielded more detections. FTA cards are suitable for collecting and transporting aMPV-positive samples, providing a reliable and hazard-free source of RNA for molecular characterization.

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          Author and article information

          Journal
          Avian Pathol.
          Avian pathology : journal of the W.V.P.A
          1465-3338
          0307-9457
          2014
          : 43
          : 2
          Affiliations
          [1 ] a Institute of Infection and Global Health , University of Liverpool , Neston , UK.
          Article
          10.1080/03079457.2014.885114
          24437352
          6d9b9920-6140-429d-b657-0aa274992064
          History

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