Disposable DNA Amplification Chips with Integrated Low-Cost Heaters  †

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Abstract

Fast point-of-use detection of, for example, early-stage zoonoses, e.g., Q-fever, bovine tuberculosis, or the Covid-19 coronavirus, is beneficial for both humans and animal husbandry as it can save lives and livestock. The latter prevents farmers from going bankrupt after a zoonoses outbreak. This paper describes the development of a fabrication process and the proof-of-principle of a disposable DNA amplification chip with an integrated heater. Based on the analysis of the milling process, metal adhesion studies, and COMSOL MultiPhysics heat transfer simulations, the first batch of chips has been fabricated and successful multiple displacement amplification reactions are performed inside these chips. This research is the first step towards the development of an early-stage zoonoses detection device. Tests with real zoonoses and DNA specific amplification reactions still need to be done.

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Author and article information

Journal

Journal ID (nlm-ta): Micromachines (Basel)

Journal ID (iso-abbrev): Micromachines (Basel)

Journal ID (publisher-id): micromachines

Title: Micromachines

Publisher: MDPI

ISSN (Electronic): 2072-666X

Publication date (Electronic): 25 February 2020

Publication date Collection: March 2020

Volume: 11

Issue: 3

Electronic Location Identifier: 238

Affiliations

[1 ]Department of Integrated Devices and Systems, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands; nanoakegawa@ 123456gmail.com (F.A.M.); r.g.p.sanders@ 123456utwente.nl (R.S.); r.j.wiegerink@ 123456utwente.nl (R.W.); j.c.lotters@ 123456utwente.nl (J.L.)

[2 ]Bronkhorst High-Tech BV, Nijverheidsstraat 1A, 7261 AK Ruurlo, The Netherlands

Author notes

[* ]Correspondence: h.veltkamp@ 123456utwente.nl ; Tel.: +31-53-489-5594

[†]

This paper is an extended version of our paper published in the 4th Microfluidic Handling Systems Conference, Enschede, The Netherlands, 2–4 of October 2019.

[‡]

These authors contributed equally to this work.

Author information

Henk-Willem Veltkamp https://orcid.org/0000-0002-6044-5891

Fernanda Akegawa Monteiro https://orcid.org/0000-0001-8095-7459

Remco Wiegerink https://orcid.org/0000-0001-5571-739X

Article

Publisher ID: micromachines-11-00238

DOI: 10.3390/mi11030238

PMC ID: 7143804

PubMed ID: 32106462

SO-VID: 6dc19825-475c-47c0-bb08-3b20128e365d

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).