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Abstract
A PCR test has been developed for the specific identification of Legionella longbeachae.
The test targeted sequence unique to both L. longbeachae serogroups 1 and 2 within
the mip gene and permitted both species and serogroup identification. The test was
trialed on a range of closely related species and 20 clinical isolates originating
from Australia, the USA and Israel. Results were consistent with previous identification
analyses. From 20 water samples known to contain Legionella spp. one sample yielded
isolates which consistently tested positive by L. longbeachae serogroup 1 PCR. DNA
sequencing of the PCR product, 5S rRNA gene sequence and hybridisation analysis with
a specific oligonucleotide probe definitively identified one isolate as L. longbeachae
serogroup 1. PCR testing was demonstrated as a superior method of identification to
traditional seroagglutination reactions, which were ambiguous and could explain the
previous failure to identify the presence of this microorganism in water.