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      Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of bla CTX-M-2 in a fosA6-Harboring Escherichia coli Sequence Type 410 Strain

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          Abstract

          The extended-spectrum β-lactamase gene bla CTX-M-2 is mainly associated with IS CR1 embedded in complex sul1-type integrons, but information on the genetic context of plasmids harboring the IS CR1-bla CTX-M-2 module remains limited. In this study, a bla CTX-M-2-harboring plasmid (pYD786-1) belonging to the sequence type 2 (ST2)-IncHI2 plasmid type and isolated from an Escherichia coli ST410 clinical strain was sequenced and analyzed. pYD786-1 belongs to the APEC-O1-R-type IncHI2 plasmids, which are widely distributed in human, poultry, and livestock strains. It contains a multidrug resistance mosaic region (MRR) consisting of a Tn 21::In2 transposon backbone augmented by acquisition of duplicate IS CR1-bla CTX-M-2 modules. Tn 2411, a Tn 21::In2 precursor, likely played a role in the generation of the MRR in pN13-01290_23, the putative progenitor plasmid of pYD786-1, found in a foodborne Salmonella strain. Tn 21/Tn 2411::In::IS CR1-bla CTX-M-2 derivatives, including pYD786-1, have been identified in strains from Europe, South America, and the United States, suggesting potential global dissemination of the bla CTX-M-2 modules mediated by this vehicle.

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          Author and article information

          Journal
          Antimicrob Agents Chemother
          Antimicrob. Agents Chemother
          aac
          aac
          AAC
          Antimicrobial Agents and Chemotherapy
          American Society for Microbiology (1752 N St., N.W., Washington, DC )
          0066-4804
          1098-6596
          29 August 2016
          21 October 2016
          November 2016
          : 60
          : 11
          : 6742-6747
          Affiliations
          [a ]Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China
          [b ]Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
          [c ]University of Limoges, INSERM, CHU Limoges, UMR_S 1092, Limoges, France
          [d ]Modernizing Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
          [e ]Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
          Author notes
          Address correspondence to Yohei Doi, yod4@ 123456pitt.edu .

          Citation Guo Q, Ding B, Jové T, Stoesser N, Cooper VS, Wang M, Doi Y. 2016. Characterization of a novel IncHI2 plasmid carrying tandem copies of bla CTX-M-2 in a fosA6-harboring Escherichia coli sequence type 410 strain. Antimicrob Agents Chemother 60:6742–6747. doi: 10.1128/AAC.01173-16.

          Author information
          http://orcid.org/0000-0002-4508-7969
          http://orcid.org/0000-0001-7726-0765
          Article
          PMC5075081 PMC5075081 5075081 01173-16
          10.1128/AAC.01173-16
          5075081
          27572405
          6e394f14-60b7-4fc2-9034-7dac23512beb
          Copyright © 2016, American Society for Microbiology. All Rights Reserved.
          History
          : 6 June 2016
          : 5 July 2016
          : 23 August 2016
          Page count
          Figures: 3, Tables: 0, Equations: 0, References: 35, Pages: 6, Words: 4504
          Funding
          Funded by: HHS | National Institutes of Health (NIH) http://dx.doi.org/10.13039/100000002
          Award ID: R01AI104895
          Award ID: R21AI123747
          Award Recipient : Yohei Doi
          Funded by: National Natural Science Foundation of China (NSFC) http://dx.doi.org/10.13039/501100001809
          Award ID: 81673479
          Award ID: 81402976
          Award ID: 81120108024
          Award Recipient : Qinglan Guo
          Funded by: Shanghai Pujiang Program
          Award ID: 16PJD010
          Award Recipient : Qinglan Guo
          Funded by: Public Health England (PHE) http://dx.doi.org/10.13039/501100002141
          Award Recipient : Nicole Stoesser
          Categories
          Mechanisms of Resistance

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