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      Role of Energy Charge and Redox State for Hepatocyte Gluconeogenesis of Acutely Uremic Rats

      ,

      Nephron

      S. Karger AG

      Liver cells, Gluconeogenesis, Ketogenesis, Energy Charge, Redox State, Uremia

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          Abstract

          Hepatocytes were isolated from rats following bilateral nephrectomy, Ureter ligation or sham Operation under sodium pentobarbital (Nembutal®) anesthesia to investigate the potential role of energy Charge and redox State for the gluconeogenetic ability of liver cells. Ketogenesis from /-serine, sodium pyruvate or dihydroxyacetone was significantly higher in hepatocytes of aeutely uremic rats indicating higher concentration of reducing equivalents in the mitochondria. During ineubation, the mitochondrial redox State characterized by β-hydroxybutyrate/acetoace-tate ratio moved into direction of reduetion in all experimental groups, whereas cytosolic redox State characterized by lactate/pyruvate ratio shifted to the oxidative State indicating lack of cytosolic reducing equivalents. Hepatocyte ATP and oxoglutarate production of ureter-ligated rats were significantly higher compared with binephrectomized or sham-operated animals independent of the Substrates used. Simultaneously, energy Charge showed values higher than 0.85 only in hepatocytes of ureter-ligated animals indicating high energy supply for energy requiring processes. We conclude that hepatic gluconeogenesis and ketogenesis of aeutely uremic rats are limited by a lack of cytosolic reducing equivalents independent of cell energy supply.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1985
          1985
          24 December 2008
          : 40
          : 2
          : 206-212
          Affiliations
          Department of Internal Medicine, University of Freiburg, FRG
          Article
          190342 Nephron 1985;40:206–212
          10.1159/000190342
          4000349
          © 1985 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          Original Paper

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