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      Role of Energy Charge and Redox State for Hepatocyte Gluconeogenesis of Acutely Uremic Rats



      S. Karger AG

      Liver cells, Gluconeogenesis, Ketogenesis, Energy Charge, Redox State, Uremia

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          Hepatocytes were isolated from rats following bilateral nephrectomy, Ureter ligation or sham Operation under sodium pentobarbital (Nembutal®) anesthesia to investigate the potential role of energy Charge and redox State for the gluconeogenetic ability of liver cells. Ketogenesis from /-serine, sodium pyruvate or dihydroxyacetone was significantly higher in hepatocytes of aeutely uremic rats indicating higher concentration of reducing equivalents in the mitochondria. During ineubation, the mitochondrial redox State characterized by β-hydroxybutyrate/acetoace-tate ratio moved into direction of reduetion in all experimental groups, whereas cytosolic redox State characterized by lactate/pyruvate ratio shifted to the oxidative State indicating lack of cytosolic reducing equivalents. Hepatocyte ATP and oxoglutarate production of ureter-ligated rats were significantly higher compared with binephrectomized or sham-operated animals independent of the Substrates used. Simultaneously, energy Charge showed values higher than 0.85 only in hepatocytes of ureter-ligated animals indicating high energy supply for energy requiring processes. We conclude that hepatic gluconeogenesis and ketogenesis of aeutely uremic rats are limited by a lack of cytosolic reducing equivalents independent of cell energy supply.

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          Author and article information

          S. Karger AG
          24 December 2008
          : 40
          : 2
          : 206-212
          Department of Internal Medicine, University of Freiburg, FRG
          190342 Nephron 1985;40:206–212
          © 1985 S. Karger AG, Basel

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          Pages: 7
          Original Paper


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