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      Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations Translated title: Estudo da densidade das células ganglionares no intestino terminal de ratos portadores de anomalia anorretal

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          Abstract

          PURPOSE: To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses’ terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.

          Translated abstract

          OBJETIVO: Estudar as células ganglionares (CG) no intestino terminal de ratos portadores de anomalia anorretal (AAR) induzida pela etilenotiouréia (ETU). MÉTODOS: Os animais foram distribuídos em três grupos: Grupo A - fetos normais, obtidos de ratas grávidas às quais não foi administrada ETU; Grupo B - fetos não portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU e Grupo C - fetos portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU. A ETU foi administrada no décimo primeiro dia de gestação na dose de 125 mg/Kg, por gavagem. As ratas foram submetidas à laparotomia e histerotomia para retirada dos fetos no vigésimo primeiro dia de gestação. O intestino terminal dos fetos foi retirado e analisado por imunohistoquímica para pesquisa de CG. RESULTADOS: Foram encontradas diferenças estatisticamente significantes entre os grupos A, B e C quanto à densidade de CG. O grupo A apresentou a maior densidade, seguida pelo grupo B, e a menor densidade foi encontrada no Grupo C. CONCLUSÃO: Existe uma menor densidade de CG no intestino terminal de ratos portadores de AAR.

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          Evidence for the presence of S-100 protein in the glial component of the human enteric nervous system.

          D Cocchia, F Michetti, L Probert … (1982)
          Article 0 comments Cited 40 times – based on 0 reviews     Review now
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            Fecal incontinence in anorectal malformations, neuropathy, and miscellaneous conditions.

            R J Rintala (2002)
            In the majority cases, fecal soiling in children is functional and usually associated with severe constipation. Fortunately, functional soiling is a self-limiting problem and usually disappears at puberty. Organic fecal incontinence is a consequence of congenital malformations affecting the anorectum, anal sphincters, or the spinal cord. Inability to control bowel function may be permanent, as in patients with myelodysplasia; self-limiting, as in patients who have fecal soiling after a pull-through operation for Hirschsprung's disease; or partial, as in many patients who have undergone repair of an anorectal malformation. The purpose of this report is to review the etiology, long-term outcome, and evolution of the management of different types of organic fecal incontinence in children. Knowledge of the pathophysiology of fecal incontinence has accumulated during recent decades, and this provides the basis of modern treatment modalities that have revolutionized treatment so that today most patients can be provided total or at least social continence from early childhood.
            Article 0 comments Cited 10 times – based on 0 reviews     Review now
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              Innervation patterns of the rectal pouch and fistula in anorectal malformations: a preliminary report.

              Alexander M. Holschneider, Benno M. Ure, Walter Pfrommer … (1996)
              The innervation patterns of the rectal pouch and fistula of 52 children with anorectal malformations were investigated. Posterior sagittal anorectoplasty was used for intermediate and high anomalies; for the latter it was combined with an abdominal approach. Perineoproctoplasty was performed for low anomalies. The specimens were investigated by acetylcholinesterase staining, lactate dehydrogenase, and succinyldehydrogenase reaction. They consisted of fistula material only in 23 patients and of parts of the rectal pouch in 29. Fourty-four patients (84.6%) had follow-up, and information of bowel movements and continence was obtained after a mean of 3.3 years. Abnormal innervation patterns were found in 96% of the specimens. All fistulas were found to be aganglionic, including the adjacent part of the rectum involving the internal sphincter equivalent. Classical aganglionosis was found in 31% of the rectal pouch specimens, hypoganglionosis in 38%, neuronal intestinal dysplasia (NID) type B in 14%, and dysganglionosis in 10%. All patients with severe constipation or soiling at the time of follow-up had some histopathological correlation. Of the 25 patients for whom the specimens had consisted of rectal pouch material, nine (31%) had severe constipation. All four patients with a low-type malformation who had follow-up and pathological innervation patterns in the rectal pouch suffered from severe constipation; this was true of only five of the 19 children with intermediate or high malformations (P < .05). However, numerous pathological innervation patterns had been identified in patients who had normal bowel function at the time of follow-up. It is concluded that partial denervation of the rectum may not be the only cause in the pathogenesis of constipation after posterior sagittal anorectoplasty and perineoproctoplasty. The high frequency of neuronal intestinal malformations in the rectal pouch may be related to the higher frequency of bowel disturbances in patients with low malformations, in whom the resection was less radical. However, the clinical course is not necessarily related to specific histopathological findings. In the authors' opinion, the recommendation to use the distal rectal pouch and parts of the fistula in the reconstruction of anorectal malformations should be reconsidered.
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                Author and article information

                Contributors
                Maurício Macedo: Role: ND
                José Luiz Martins: Role: ND
                Karine Furtado Meyer: Role: ND
                Iberê Cauduro Soares: Role: ND
                Journal
                Journal ID (publisher): acb
                Title: Acta Cirurgica Brasileira
                Abbreviated Title: Acta Cir. Bras.
                Publisher: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (São Paulo )
                ISSN: 1678-2674
                Publication date (Print): December 2007
                Volume: 22
                Issue: 6
                Pages: 441-445
                Affiliations
                [1 ] Universidade Federal de São Paulo Brazil
                [2 ] Universidade Federal de São Paulo Brazil
                [3 ] Hospital of São Paulo (HSPE) Brazil
                Article
                Publisher ID: S0102-86502007000600005
                DOI: 10.1590/S0102-86502007000600005
                SO-VID: 6e82826d-6103-4a6f-aab8-50f39fb91907
                License:

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0102-8650&lng=en
                Categories
                Subject: SURGERY

                ScienceOpen disciplines: Surgery
                Keywords: Anus, Imperforate,Constipation,Ethylenethiourea,S100 protein,Enteric Nervous System,Rats,Constipação Intestinal,Etilenotiouréia,Proteínas S100,Sistema nervoso entérico,Ratos
                Data availability:
                ScienceOpen disciplines: Surgery
                Keywords: Anus, Imperforate, Constipation, Ethylenethiourea, S100 protein, Enteric Nervous System, Rats, Constipação Intestinal, Etilenotiouréia, Proteínas S100, Sistema nervoso entérico, Ratos

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