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      A Method to Quantify Neovascularization in the Mouse Cornea

      Ophthalmic Research

      S. Karger AG

      Neovascularization, Murine cornea, Angiogenesis

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          Abstract

          A method for microscopical observation and quantification of corneal neovascularization in the living mouse is described. Measurements of the corneal vasculature were made on still-mode video-pictures of the cornea. The validity of the method was shown by the high correlation between the morphometric measurements and computerized values on the same vessel structures. The method was applied to describe the neovascular response of heat-killed tumour fragments implanted into corneal pockets. The neovascular response that was observed in some corneas was dependent on the distance from the edge of the pocket with heat-killed material to the nearest vessel loop. The pocket area was vascularized only if this distance (measured the 3rd day after implantation) was less than 0.70 mm. Resorption of heat-killed tissue occurred in corneas without any neovascular response and it was not altered by penetration of vessel into the cornea. The described method proved to be suitable for studying the neovascularization induced by corneal pocket implants.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1991
          1991
          10 December 2009
          : 23
          : 2
          : 104-114
          Affiliations
          Institute for Surgical Research, University of Oslo, Rikshospitalet – The National Hospital, Oslo, Norway
          Article
          267097 Ophthalmic Res 1991;23:104–114
          10.1159/000267097
          1714560
          © 1991 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 11
          Categories
          Original Paper

          Vision sciences, Ophthalmology & Optometry, Pathology

          Angiogenesis, Murine cornea, Neovascularization

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