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      In vitro Characterization of a Membrane-Based Low-Density Lipoprotein Affinity Adsorption Device

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          The objective of this study was to explore the use of microporous membranes as an alternative substrate to porous beads in affinity adsorption of low-density lipoprotein (LDL) for therapeutic purposes. Flat sheet immunoaffinity membranes containing a polyclonal antibody preparation were utilized as the affinity substrate. The antibody was covalently immobilized to the surface through a poly(ethylene glycol) (PEG) spacer. Equilibrium adsorption of LDL from plasma was measured. Adsorption from plasma and elution of bound LDL using citrate buffer were studied as a function of flow rate.Specific capacity was as great as 2 mg apolipoprotein B per milliliter membrane volume. The superior transport properties of the membrane allowed rapid adsorption and regeneration, which translated to a large number of adsorptive cycles that can be performed within a given treatment time. On the basis of in vitro performance characteristics, it is estimated that an immunoaffinity membrane device can provide a reduction in patient plasma LDL concentration comparable to that provided by packed columns, but with almost an 80% reduction in the device volume.

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          Studies of a novel membrane for affinity separations

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            Selective in vivo removal of rheumatoid factor by an extracorporeal treatment device in rheumatoid arthritis patients

             S Saal,  S Hinkle,  SB Sorin (1991)
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              Humoral immune response following extracorporeal immunoadsorption therapy of patients with hypercholesterolemia


                Author and article information

                Blood Purif
                Blood Purification
                S. Karger AG
                June 1998
                08 July 1998
                : 16
                : 3
                : 123-134
                University of Wisconsin, Madison, Wisc., USA
                14325 Blood Purif 1998;16:123–134
                © 1998 S. Karger AG, Basel

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                Pages: 12
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