Lumacaftor (VX-809) restores the ability of CF macrophages to phagocytose and kill Pseudomonas aeruginosa

Abstract

<p class="first" id="d7475079e221">Cystic fibrosis (CF), the most common lethal genetic disease in Caucasians, is characterized by chronic bacterial lung infection and excessive inflammation, which lead to progressive loss of lung function and premature death. Although ivacaftor (VX-770) alone and ivacaftor in combination with lumacaftor (VX-809) improve lung function in CF patients with the <i>Gly551Asp</i> and <i>del508Phe</i> mutations, respectively, the effects of these drugs on the function of human CF macrophages are unknown. Thus studies were conducted to examine the effects of lumacaftor alone and lumacaftor in combination with ivacaftor (i.e., ORKAMBI) on the ability of human CF ( <i>del508Phe</i>/ <i>del508Phe</i>) monocyte-derived macrophages (MDMs) to phagocytose and kill <i>Pseudomonas aeruginosa</i>. Lumacaftor alone restored the ability of CF MDMs to phagocytose and kill <i>P. aeruginosa</i> to levels observed in MDMs obtained from non-CF (WT-CFTR) donors. This effect contrasts with the partial (~15%) correction of del508Phe Cl ⁻ secretion of airway epithelial cells by lumacaftor. Ivacaftor reduced the ability of lumacaftor to stimulate phagocytosis and killing of <i>P. aeruginosa</i>. Lumacaftor had no effect on <i>P. aeruginosa-</i>stimulated cytokine secretion by CF MDMs. Ivacaftor (5 µM) alone and ivacaftor in combination with lumacaftor reduced secretion of several proinflammatory cytokines. The clinical efficacy of ORKAMBI may be related in part to the ability of lumacaftor to stimulate phagocytosis and killing of <i>P. aeruginosa</i> by macrophages. </p>