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      Purification of dorsal root ganglion neurons from rat by immunopanning.

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      Cold Spring Harbor protocols
      Cold Spring Harbor Laboratory

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          Abstract

          Dorsal root ganglion neurons (DRGs) are sensory neurons that facilitate somatosensation and have been used to study neurite outgrowth, regeneration, and degeneration and PNS and CNS myelination. Studies of DRGs have relied on cell isolation strategies that generally involve extended culture in the presence of antimitotic agents or other cytotoxic treatments that target dividing cells. The surviving cells typically are dependent on serum for growth. Other methods, involving purification of DRGs based on their large size, produce low yield. In contrast, the immunopanning-based method described here for prospective isolation of DRGs from rodents allows for rapid purification in the absence of antimitotic agents and serum. These DRG cultures take place in a defined medium. They are free of Schwann cells and other glia and thus can be used to study the role of glia in the biology of DRG neurons.

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          Author and article information

          Journal
          Cold Spring Harb Protoc
          Cold Spring Harbor protocols
          Cold Spring Harbor Laboratory
          1559-6095
          1559-6095
          Aug 01 2014
          : 2014
          : 8
          Affiliations
          [1 ] Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305.
          Article
          2014/8/pdb.prot074948 NIHMS689169
          10.1101/pdb.prot074948
          4438770
          25086011
          6f636667-678c-4c5b-bea8-bd4319290cc7
          History

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