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      Co-suppression of AtMIPS demonstrates cooperation of MIPS1, MIPS2 and MIPS3 in maintaining myo-inositol synthesis.

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          Abstract

          Co-suppressed MIPS2 transgenic lines allow bypass of the embryo lethal phenotype of the previously published triple knock-out and demonstrate the effects of MIPS on later stages of development. Regulation of inositol production is of interest broadly for its effects on plant growth and development. The enzyme L-myo-inositol 1-phosphate synthase (MIPS, also known as IPS) isomerizes D-glucose-6-P to D-inositol 3-P, and this is the rate-limiting step in inositol production. In Arabidopsis thaliana, the MIPS enzyme is encoded by three different genes, (AtMIPS1, AtMIPS2 and AtMIPS3), each of which has been shown to produce proteins with biochemically similar properties but differential expression patterns. Here, we report phenotypic and biochemical effects of MIPS co-suppression. We show that some plants engineered to overexpress MIPS2 in fact show reduced expression of AtMIPS1, AtMIPS2 and AtMIPS3, and show altered vegetative phenotype, reduced size and root length, and delayed flowering. Additionally, these plants show reduced inositol, increased glucose levels, and alteration of other metabolites. Our results suggest that the three AtMIPS genes work together to impact the overall synthesis of myo-inositol and overall inositol homeostasis.

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          Author and article information

          Journal
          Plant Mol Biol
          Plant molecular biology
          Springer Science and Business Media LLC
          1573-5028
          0167-4412
          Jun 2018
          : 97
          : 3
          Affiliations
          [1 ] Biology Department, Emory & Henry College, Emory, VA, 24327, USA. cfleet@ehc.edu.
          [2 ] Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.
          [3 ] Department of Plant Biology, Carnegie Institution for Science, 260 Panama Street, Stanford, CA, 93405, USA.
          [4 ] Biology Department, Emory & Henry College, Emory, VA, 24327, USA.
          [5 ] Lincoln Memorial University College of Veterinary Medicine, Harrogate, TN, 37752, USA.
          [6 ] Department of Biochemistry, Virginia Tech, Blacksburg, VA, 24061, USA.
          Article
          10.1007/s11103-018-0737-6
          10.1007/s11103-018-0737-6
          29777485
          6fec2cb6-d967-4795-a311-c30514d38101
          History

          Inositol,Co-suppression,Arabidopsis thaliana,L-myo-inositol 1-phosphate synthase

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