For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
186
views
279
references
 Top references
cited by
98
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    8
    shares
      283
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Learning from Bacteriophages - Advantages and Limitations of Phage and Phage-Encoded Protein Applications

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The emergence of bacteria resistance to most of the currently available antibiotics has become a critical therapeutic problem. The bacteria causing both hospital and community-acquired infections are most often multidrug resistant. In view of the alarming level of antibiotic resistance between bacterial species and difficulties with treatment, alternative or supportive antibacterial cure has to be developed. The presented review focuses on the major characteristics of bacteriophages and phage-encoded proteins affecting their usefulness as antimicrobial agents. We discuss several issues such as mode of action, pharmacodynamics, pharmacokinetics, resistance and manufacturing aspects of bacteriophages and phage-encoded proteins application.

          Related collections

          Most cited references279

          • Record: found
          • Abstract: found
          • Article: not found

          Dispersing biofilms with engineered enzymatic bacteriophage.

          Timothy K. Lu, James J. Collins (2007)
          Synthetic biology involves the engineering of biological organisms by using modular and generalizable designs with the ultimate goal of developing useful solutions to real-world problems. One such problem involves bacterial biofilms, which are crucial in the pathogenesis of many clinically important infections and are difficult to eradicate because they exhibit resistance to antimicrobial treatments and removal by host immune systems. To address this issue, we engineered bacteriophage to express a biofilm-degrading enzyme during infection to simultaneously attack the bacterial cells in the biofilm and the biofilm matrix, which is composed of extracellular polymeric substances. We show that the efficacy of biofilm removal by this two-pronged enzymatic bacteriophage strategy is significantly greater than that of nonenzymatic bacteriophage treatment. Our engineered enzymatic phage substantially reduced bacterial biofilm cell counts by approximately 4.5 orders of magnitude ( approximately 99.997% removal), which was about two orders of magnitude better than that of nonenzymatic phage. This work demonstrates the feasibility and benefits of using engineered enzymatic bacteriophage to reduce bacterial biofilms and the applicability of synthetic biology to an important medical and industrial problem.
          Article 0 comments Cited 238 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            5500 Phages examined in the electron microscope.

            H.-W. Ackermann (2007)
            "Phages" include viruses of eubacteria and archaea. At least 5568 phages have been examined in the electron microscope since the introduction of negative staining in 1959. Most virions (96%) are tailed. Only 208 phages (3.7%) are polyhedral, filamentous, or pleomorphic. Phages belong to one order, 17 families, and three "floating" groups. Phages are found in 11 eubacterial and archaeal phyla and infect 154 host genera, mostly of the phyla Actinobacteria, Firmicutes, and Proteobacteria. Of the tailed phages, 61% have long, noncontractile tails and belong to the family Siphoviridae. Convergent evolution is visible in the morphology of certain phage groups.
            Article 0 comments Cited 211 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              The phage-host arms race: shaping the evolution of microbes.

              Adi Stern, Rotem Sorek (2011)
              Bacteria, the most abundant organisms on the planet, are outnumbered by a factor of 10 to 1 by phages that infect them. Faced with the rapid evolution and turnover of phage particles, bacteria have evolved various mechanisms to evade phage infection and killing, leading to an evolutionary arms race. The extensive co-evolution of both phage and host has resulted in considerable diversity on the part of both bacterial and phage defensive and offensive strategies. Here, we discuss the unique and common features of phage resistance mechanisms and their role in global biodiversity. The commonalities between defense mechanisms suggest avenues for the discovery of novel forms of these mechanisms based on their evolutionary traits.
              Article 0 comments Cited 204 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Curr Protein Pept Sci
                Journal ID (iso-abbrev): Curr. Protein Pept. Sci
                Journal ID (publisher-id): CPPS
                Title: Current Protein & Peptide Science
                Publisher: Bentham Science Publishers
                ISSN (Print): 1389-2037
                ISSN (Electronic): 1875-5550
                Publication date (Print): December 2012
                Publication date (Electronic): December 2012
                Volume: 13
                Issue: 8
                Pages: 699-722
                Affiliations
                [1 ]Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland
                [2 ]Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, box 2462, B-3001 Leuven, Belgium
                Author notes
                [* ]Address correspondence to this author at the Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland; Tel:/Fax: +48 71 325 21 51; Email: zuzanna.drulis-kawa@ 123456microb.uni.wroc.pl
                Article
                Publisher ID: CPPS-13-699
                DOI: 10.2174/138920312804871193
                PMC ID: 3594737
                PubMed ID: 23305359
                SO-VID: 70274b09-b0c9-4682-8dd1-57aec87396e2
                Copyright © © 2012 Bentham Science Publishers
                License:

                This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 18 June 2012
                Date revision received : 12 July 2012
                Date accepted : 20 September 2012
                Categories
                Subject: Article

                ScienceOpen disciplines: Biochemistry
                Keywords: antibiotics,bacterial multidrug resistance,bacteriophage therapy,phage-encoded proteins application,
                Data availability:
                ScienceOpen disciplines: Biochemistry
                Keywords: antibiotics, bacterial multidrug resistance, bacteriophage therapy, phage-encoded proteins application,

                Comments

                Comment on this article

                scite_

                Similar content283

                See all similar

                Cited by97

                See all cited by

                Most referenced authors1,634

                See all reference authors