Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS ONE
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
30
June
2016
Publication date Collection: 2016
Volume: 11
Issue: 6
Electronic Location Identifier: e0157366
Affiliations
[1
]Hydrosystems and Bioprocesses Research unit, IRSTEA, Antony, France
[2
]SPYGEN, Le Bourget du Lac, France
[3
]Rhône-Alpes Regional Direction, ONEMA, Bron, France
[4
]Laboratoire d'Ecologie Alpine (LECA), CNRS, Grenoble, France
[5
]Laboratoire d'Ecologie Alpine (LECA), Université Grenoble-Alpes, Grenoble, France
Swansea University, UNITED KINGDOM
Author notes
Competing Interests: The authors have read the journal's policy and the authors of this manuscript have
the following competing interests: PT is co-inventor of a patent on "teleo" primers
and on the use of the amplified fragment for identifying fish species from environmental
samples. This patent only restricts commercial applications and has no impact on the
use of this method by academic researchers. AV and TD are research scientists in a
private company (SPYGEN), specialized on the use of eDNA for species detection. The
private companies EDF and SPYGEN participated at this study, but this does not alter
the authors' adherence to PLOS ONE policies on sharing data and materials.
Conceived and designed the experiments: RC AV TD JCR NR DP. Performed the experiments:
RC TD JCR NR. Analyzed the data: RC JCR NR DP. Wrote the paper: RC AV TD JCR NR AB
PT DP. Provided guidance during the study: AV PT DP. Performed the metabarcoding analysis:
RC AV TD.
Article
Publisher ID:
PONE-D-15-54691
DOI: 10.1371/journal.pone.0157366
PMC ID: 4928825
PubMed ID: 27359116
SO-VID: 703657ec-fd40-4875-9b52-125256b64193
Copyright © © 2016 Civade et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
In addition to their participation to field work, data collection and preparation
of the manuscript, ONEMA (French National Agency for Water and Aquatic Environments)
and SPYGEN financially supported this work (Contract IRSTEA-SPYGENONEMA, 2012-2015).
EDF (Electricity of France) also supported financially this study. The funders had
no role in study design, data collection and analysis, decision to publish, or preparation
of the manuscript.
Subject:
Research Article
Subject:
Earth Sciences
Subject:
Marine and Aquatic Sciences
Subject:
Bodies of Water
Subject:
Lakes
Subject:
Ecology and Environmental Sciences
Subject:
Aquatic Environments
Subject:
Freshwater Environments
Subject:
Lakes
Subject:
Earth Sciences
Subject:
Marine and Aquatic Sciences
Subject:
Aquatic Environments
Subject:
Freshwater Environments
Subject:
Lakes
Subject:
Earth Sciences
Subject:
Marine and Aquatic Sciences
Subject:
Bodies of Water
Subject:
Rivers
Subject:
Ecology and Environmental Sciences
Subject:
Aquatic Environments
Subject:
Freshwater Environments
Subject:
Rivers
Subject:
Earth Sciences
Subject:
Marine and Aquatic Sciences
Subject:
Aquatic Environments
Subject:
Freshwater Environments
Subject:
Rivers
Subject:
Biology and Life Sciences
Subject:
Organisms
Subject:
Animals
Subject:
Vertebrates
Subject:
Fishes
Subject:
Freshwater Fish
Subject:
Earth Sciences
Subject:
Hydrology
Subject:
Surface Water
Subject:
Biology and Life Sciences
Subject:
Organisms
Subject:
Animals
Subject:
Vertebrates
Subject:
Fishes
Subject:
Osteichthyes
Subject:
Trout
Subject:
Biology and Life Sciences
Subject:
Ecology
Subject:
Biodiversity
Subject:
Ecology and Environmental Sciences
Subject:
Ecology
Subject:
Biodiversity
Subject:
Earth Sciences
Subject:
Hydrology
Subject:
Fresh Water
Subject:
Research and Analysis Methods
Subject:
Database and Informatics Methods
Subject:
Biological Databases
Subject:
Sequence Databases
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Sequencing Techniques
Subject:
Sequence Analysis
Subject:
Sequence Databases
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Sequencing Techniques
Subject:
Sequence Analysis
Subject:
Sequence Databases