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      A novel endogenous induction of ColE7 expression in a csrA mutant of Escherichia coli.

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          Abstract

          Carbon storage regulator A (CsrA) is an important regulator that controls central metabolic pathways and a variety of physiological functions. We found that disruption of csrA in cells containing the ColE7 operon caused a 12-fold increase in colicin E7 production. Moreover, real-time RT-PCR demonstrated a decrease of around 50 % in the lexA mRNA of the csrA mutant. However, the cellular level of RecA protein and its mRNA were not significantly different from the wild type strain. Our results suggest that a novel induction mechanism might exist in E. coli that allows the expression of ColE7 operon in response to a metabolic shift. Proteomic analysis suggested that csrA deficient mutant may adapt PEP-glyoxylate cycle for energy production. Thus, the physiological changes in the csrA mutant may be similar to carbon source limitation for initiating the expression of ColE7 operon in response to stringent environmental conditions.

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          Author and article information

          Journal
          Curr. Microbiol.
          Current microbiology
          Springer Nature America, Inc
          1432-0991
          0343-8651
          Apr 2013
          : 66
          : 4
          Affiliations
          [1 ] Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, 11221, Taiwan.
          Article
          10.1007/s00284-012-0282-5
          23247769
          70b312d6-9018-40a7-8186-57fd3fe1dcf7
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