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Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.

Author(s): M Bateson, Jordan D. Ward, E D Kopczynski

Publication date: 1994-01-31

Journal: Applied and Environmental Microbiology

Keywords: Base Sequence, Chimera, genetics, Clone Cells, Cyanobacteria, DNA, Fungal, isolation & purification, DNA, Ribosomal, Molecular Sequence Data

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Abstract

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

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PubMed ID:: 8135524

PMC ID:: 201378

DOI:: 10.1128/aem.60.2.746-748.1994

Keywords: Base Sequence,Chimera,genetics,Clone Cells,Cyanobacteria,DNA, Fungal,isolation & purification,DNA, Ribosomal,Molecular Sequence Data

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Keywords: Base Sequence, Chimera, genetics, Clone Cells, Cyanobacteria, DNA, Fungal, isolation & purification, DNA, Ribosomal, Molecular Sequence Data

Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.

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